Summary
A computer-assisted on-line glucose analyzer was developed for feed-back control of cell growth. Using this system the glucose consumption rate for Escherichia coli was determined to be linear during batch culture at 0.37 g/hr. On-line feed-back control of glucose concentration at 1.5±0.5 g/L was used with fed-batch cultures to produce 31.2 g dry weight of E. coli cells/L in 12 h.
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Allen, B.R., and Luli, G.W. (1985). The World Biotechnology Report. Proc. Biotech. '85 (USA) 2, 447–466.
Cleland, N., and Enfors, S. (1983). Eur. J. Appl. Microbiol. Biotechnol. 18, 141–147.
Ghoul, M., Ronat, E., and Engasser, J. (1986). Biotechnol. Bioeng. 28, 119–121.
Pan, J.G., Rhee, J.S., and Lebault, J.M. (1987). Biotechnol. Lett. 9, 89–94.
Parker, C.P., Gardell, M.G., and DiBiasio, D. (1985). Amer. Biotechnol. Lab. Sept/Oct, pp. 37–46.
Reiling, H.E., Laurila, H., and Fiechter, A. (1985). J. Biotechnol. 2, 191–206.
Shay, L.K., Hunt, H.R., and Wegner, G.H. (1987). J. Industr. Microbiol. 2, 79–85.
Strohl, W.R., Schlasner, S.M., and Lorensen, P.L. (1986). Biotechniques 4, 336–343.
Suzuki, T., Yamane, T., and Shimizu, S. (1987). Appl. Microbol. Biotechnol. 25, 526–531.
Titus, J.A., Luli, G.W., Dekleva, M.L., and Strohl, W.R. (1984). Appl. Environ. Microbiol. 47, 239–244.
Zabriskie, D.W., Wareheim, D.A., and Polansky, M.J. (1987). J. Industr. Microbiol. 2, 87–95.
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Luli, G.W., Schlasner, S.M., Ordaz, D.E. et al. An automatic, on-line glucose analyzer for feed-back control of fed-batch growth of Escherichia coli . Biotechnol Tech 1, 225–230 (1987). https://doi.org/10.1007/BF00155459
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DOI: https://doi.org/10.1007/BF00155459