Summary
Plasmid, pBI221, was introduced into protoplasts of Chlorella saccharophila c-211-1a prepared from the cells in the stationary phase by electroporation. Transient expression of the introduced plasmid was observed under a field strength of between 600 and 900 V/cm, and a pulse duration of around 400 ms, where high membrane permeability to 70-kDa FITC-dextran was ascertained.
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Maruyama, M., Horáková, I., Honda, H. et al. Introduction of foreign DNA into Chlorella saccharophila by electroporation. Biotechnol Tech 8, 821–826 (1994). https://doi.org/10.1007/BF00152891
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DOI: https://doi.org/10.1007/BF00152891