Abstract
Plant cells obtained from suspension cultures of Catharanthus roseus were immobilized in agarose beads or in a polyurethane matrix. Permeabilization of these cells with dimethylsulf-oxide allowed measurement of the enzymes isocitrate dehydrogenase (primary metabolism) and cathenamine reductase(secondary metabolism). Enhanced storage and operational stability was achieved by treating the cells with hardening agent glutardialdehyde in combination with hexamethylenediamine.
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Felix, H.R., Mosbach, K. Enhanced stability of enzymes in permeabilized and immobilized cells. Biotechnol Lett 4, 181–186 (1982). https://doi.org/10.1007/BF00144321
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DOI: https://doi.org/10.1007/BF00144321