Abstract
From low-iron cultures of Acinetobacter haemolyticus ATCC 17906, a new hydroxamate siderophore was purified by XAD-7 adsorption followed by preparative thin layer chromatography. The siderophore, named acinetoferrin, released citric acid, 1,3-diaminopropane and (E)-2-octenoic acid upon hydrolysis with HCl, reductive hydrolysis with HI and oxidation with periodate, respectively. Structure elucidation by a combination of NMR spectroscopy and positive fast atom bombardment mass spectrometry revealed that acinetoferrin is a derivative of citric acid, both of its terminal carboxyl groups being symmetrically amide-linked with the 1-amino-3-(N-hydroxy-N-2-octenylamino)propane residues. The (E)-2-octenoic acid is novel as a component of the siderophores.
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Okujo, N., Sakakibara, Y., Yoshida, T. et al. Structure of acinetoferrin, a new citrate-based dihydroxamate siderophore fromAcinetobacter haemolyticus . Biometals 7, 170–176 (1994). https://doi.org/10.1007/BF00140488
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DOI: https://doi.org/10.1007/BF00140488