Summary
The effects of glucose on biomass and temperature-mediated recombinant gene expression has been studied in Escherichia coli batch cultures. Glucose, above 2 g/L, has inhibitory effects on aerobic cell growth and specially on gene expression. Although lower concentrations have no influences on biomass, a tenuous, but still remarkable concentration-dependent reduction of gene expression levels was observed even at about 0.1 g/L.
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Vila, P., Villaverde, A. Inhibition of CI857-controlled recombinant gene expression in Escherichia coli at very low concentrations of glucose. Biotechnol Lett 15, 549–552 (1993). https://doi.org/10.1007/BF00138537
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DOI: https://doi.org/10.1007/BF00138537