Summary
A preliminary test for the enzymatic conversion of D-xylose into xylitol by the intact cells of Candida pelliculosa (xylose reductase) coupled with the intact cells of a formate-utilizing methanogen strain HU (hydrogenase and F420-NADP oxidoreductase) was conducted by using H2 as an electron donor of NADP+. In the system, NADP(H) was well regenerated via the methanogen cells and about 90% conversion of xylose to xylitol (ca. 8 g/l) could be achieved at 35°C and pH 7.5 after 24 h incubation.
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Kitpreechavanich, V., Hayashi, M., Nishio, N. et al. Conversion of D-xylose into xylitol by xylose reductase from Candida pelliculosa coupled with the oxidoreductase system of methanogen strain HU. Biotechnol Lett 6, 651–656 (1984). https://doi.org/10.1007/BF00133831
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DOI: https://doi.org/10.1007/BF00133831