Summary
Plant hairy root cultures of Lithospermum erythrorhizon were carried out to produce shikonin derivatives by employing in situ extraction with n-hexadecane in a shake flask and a bubble column bioreactor. Over 95 % shikonin produced was recovered in the n-hexadecane layer. In flask cultures the maximum concentration of shikonin with n-hexadecane extraction was 3 times higher than that obtained without extraction. In the two phase bubble column reactor, 572.6 mg/L of shikonin and 15.6 g/L of dry cell mass were obtained after 54 days. Shikonin was produced at a constant level of 10.6 mg/L day during this period.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Byun, S.Y., Pedersen, H., and Chin, C. (1990) Phytochem., 29, 3135–3139.
Deno, H., Suga, C., Morimoto, T., and Fujita, Y. (1987). Plant Cell Reports 6, 197–199.
Fujita, Y., and Tabata, M. (1987) In: Plant Tissue and Cell Culture (ed. Green, C.E., Somers, D.A., Hackett, W.P., Biesboer, D.D.), 169–185, New York: Liss.
Green, K.D., Thomas, N.H., and Callow, J.A. (1992) Biotechnol. Bioeng., 39, 195–202.
Hamill, J.D., Parr, A.J., Rhodes, M.J.C., Robins, R.J., and Walton, N.J. (1987) Bio/Technology, 5, 800–804.
Kim, D.J. and Chang, H.N. (1990) Biotechnol. Bioeng., 36, 460–466.
Nikolova, P., Moo-Young, M., and Legge, R.L. (1991) Plant Cell Tissue and Organ Culture, 25, 219–224.
Schenk, R.U., and Hildebrandt, A.C. (1972) Can. J. Bot., 50, 199–202.
Seo, W.T., Park, Y.H., and Choe, T.B. (1992) J. Microbiol. Biotechnol. 2, 41–45.
Shimomura, K., Sudo, H., Saga, H., and Kamada, H. (1991) Plant Cell Reports 10, 282–285.
Singleton, V.L. and Rossi, J.A. (1965) Am. J. Enol. Vitic., 163, 144–158.
Taya, M., Yoyoma, A., Kondo, O., Kobayashi, T., and Matsui, C. (1989) J. Chem Eng. Japan 22, 84–89.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sim, S.J., Chang, H.N. Increased shikonin production by hairy roots of Lithospermum erythrorhizon in two phase bubble column reactor. Biotechnol Lett 15, 145–150 (1993). https://doi.org/10.1007/BF00133014
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00133014