Summary
The immobilization of the mycelial-associated β-glucosidase of Trichoderma E-58 has been carried out by encapsulating, in calcium alginate beads, the fungal mycelium obtained durinq liquid culture. The activity of this immobilized β-glucosidase was found to vary with culture age and to be more thermally stable than the extracellular β-glucosidase produced by this organism. The activity of the immobilized enzyme was successfully demonstrated in both static and shake-flask batch reaction mixtures at 50°C using both cellobiose and salicin as substrates.
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Matteau, P.P., Saddler, J.N. Glucose production using immobilized mycelial-associated β-glucosidase of Trichoderma E58. Biotechnol Lett 4, 513–518 (1982). https://doi.org/10.1007/BF00131574
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DOI: https://doi.org/10.1007/BF00131574