Summary
A novel two enzyme system of fructosyltransferase and glucose oxidase to enhance the content of the net fructo—oligosaccharide (FOS) fractions in the industrial production of FOS syrup from sucrose was devised. The net FOS content in the commercial FOS syrup has been limited only to 55–60 % due to the accumulation of glucose which acts as a feedback inhibitor of the fructosyltransferase. By supplementing glucose oxidase to the conventional FOS reaction system, we could convert the glucose to gluconic acid readily separable from neutral sugars by simple ion exchange operation in the next step. The simultaneous removal of glucose was proved effective in proceeding the reaction by fructosyltransferase further by relieving the product inhibition caused by glucose. By this way, we could raise the net FOS content as high as 90 %.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Adachi, T. (1983). Japan Food Sci, 22, 71–78.
Gupta, A.K. and Bhatia, I.S. (1982). Psytochemistry, 21, 1249–1253.
Hidaka, H., Eida, T., Tokunaga, T., and Tashiro, Y. (1986). Bifidobacteria Microflora, 5, 37–42.
Hirota, T., Shioda, K., Ishikawa, H., and Ando, M. (1981). J. Chem. Eng. Jpn., 45, 41–50.
Jung, K.H., Lim, J.Y., Yoo, S.J., Lee, J.H., and Yoo, M.Y. (1987). Biotechnol. Lett., 9, 703–708.
Jung, K.H., Yun, J.W., Kang, K.R., Lim, J.Y., and Lee, J.H. (1989). Enzyme Microbial Technol., 11, 491–494.
Kobayashi, T., Ban, T., Shimizu, S., Ohmiya, K., and Shimizu, S. (1978). J. Ferment. Technol., 56 (5), 506–510.
Oku, T., Tokunoga T., and Hosoya, N. (1984). J. Nutr., 114, 1574–1578.
Reed, G. (1975). Enzymes in Food Processing, pp. 519–547, Academic Press.
Smith, J.A., Grove, D., Luenser, S.J., and Park, L.G. (1982). US Patent, 4,309,505.
Yun, J.W., Jung, K.H., Jeon, Y.J., and Lee, J.H. (1992). J. Microbiol. Biotechnol., 2 (2), 98–101.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hoon Jung, K., Hwan Kim, J., Joong Jeon, Y. et al. Production of high fructo-oligosaccharide syrup with two enzyme system of fructosyltransferase and glucose oxidase. Biotechnol Lett 15, 65–70 (1993). https://doi.org/10.1007/BF00131555
Issue Date:
DOI: https://doi.org/10.1007/BF00131555