Summary
The mycelium-bound lipase of Aspergillus flavus was stabilised by cross-linkage using glutaraldehyde or methylglyoxal. Lipase activity was enhanced by up to 48% by treatment with methylglyoxal but not by glutaraldehyde. Cross-linking with methylglyoxal increased the thermal stability of the lipase by 58% at 50 °C
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abdul-Naby, M.A.(1993) Appl. Biochem. Biotechnol., 38, 64–81.
Avreamas, S. & Ternynck, T. (1969) Immunochemistry, 6, 53–66.
Carrara, C.R. & Rubiolo, A.C. (1994) Biotechnol. Prog., 10, 220–224.
Druet, D., El Abbadi, N. & Comeau, C.C. (1992) Appl. Microbiol. Biotechnol. 37, 745–749.
Jang, K.H., Park, C.J. & Chan, U.H. (1992) Biotechnol. Lett., 14, 311–316.
Kilara, A., Shahani, K.M. & Wagner, F.W. (1977) Biotechnol. Bioeng. 19, 1703–1714.
Kosugi, Y. & Suzuki, H. (1992) Biotechnol. Bioeng. 40, 369–374.
Long, K., Ghazali, H.M., Ariff, A., Ampon, K. & Bucke, C. (1996) J.Chem.Tech. Biotechnol. 67, in press.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.
Omar, I.C., Saeki, H., Nishio, N. & Nagai, S. (1988) Agric. Biol. Chem. 52 99–105.
Shaw, J.F., Chang, R.C., Wang, G.G. & Wang, Y.J. (1990) Biotechnol. Bioeng. 35, 132–137.
Stark, M.B. & Holmberg, K. (1989) Biotechnol. Bioeng. 34, 942–950.
Sugiura, T., Ota, Y. & Minoda, Y. (1975) Agric. Biol. Chem. 39, 1689–1694. Torchilin, V.P., Maksimenko, A.V., Klibanov, A.M., Berezin, I.V. & Martinek, K. (1978) Biochim. Biophys. Acta 522, 272–283.
Torchilin, V.P., Trubetskoy, V.S. & Martinek, K. (1983) Mol. Catal. 19, 291–307.
Wong, S.S. & Wong, L.C. (1992) Enzyme Microb. Technol., 14 866–874.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Long, K., Ghazali, H.M., Ariff, A. et al. In-situ crosslinking of Aspergillus flavus lipase: Improvement of activity, stability and properties. Biotechnol Lett 18, 1169–1174 (1996). https://doi.org/10.1007/BF00128587
Issue Date:
DOI: https://doi.org/10.1007/BF00128587