Summary
Production of anthocyanin was greater in conditioned medium than in LS medium. The highest production was obtained after 2 days culturing, and the value reached 1100 μg/g cell, which was about twice that of LS medium cultured in each cell inoculum. The highest anthocyanin content was obtained with a 1g cell (fresh weight) inoculation using conditioned medium. The percentage of the main anthocyanin, peonidin-3-glucoside, increased in accordance with the decrease in cell inoculum size, contrary to the results obtained for cyanidin-3-glucoside. This tendency was more clearly observed in LS medium than in conditioned medium.
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Igarashi K, Abe S, Sato J. (1990) Agric. Biol. Chem. 54, 171–175.
Igarashi K, Inagaki K. (1991) Agric. Biol. Chem. 55, 285–287.
Kamei, H. Kojima, S. Hasegawa, M. Umeda,T. Terabe,K. and Yukawa, T. (1993) J. Clini Exp Med. 164, 829–830.
Linsmaier, E.M. and Skoog, F. (1965) Physiol. Plant. 18, 100–127.
Mori, T. Sakurai, M. Shigeta, J. Yoshida, K and Kondo, T. (1993) J. Food Sci. 58, 788–792.
Mori, T. Sakurai, M. (1994) J. Food Sci. 59, 588–594.
Mori, T. Sakurai, M. Seki, M. and Furusaki, S. (1994a) J. Sci. Fd Agric. 65, 271–276.
Mori, T. Sakurai, M. Seki, M. and Furusaki, S. (1994b) J. Sci. Fd and Agric. 66, 381–388.
Stuart, R., Street, H. E. (1969) J Exp Bot. 20, 556–571.
Stuart, R., Street, H. E. (1971) J Exp Bot. 22, 96–106.
Yamakawa, T., Onomichi, K., Kodama, T., Minoda, Y. (1985) Agric Biol Chem. 49, 3583–3585.
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Sakurai, M., Mori, T., Seki, M. et al. Changes of anthocyanin composition by conditioned medium and cell inoculum size using strawberry suspension culture. Biotechnol Lett 18, 1149–1154 (1996). https://doi.org/10.1007/BF00128583
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DOI: https://doi.org/10.1007/BF00128583