Summary
A specific 1,2-α-mannosidase was isolated from A. phoenicis and used in the equilibrium-controlled synthesis of α1→2-linked mannobiose and mannotriose. Yields of 22.33 % disaccharide, 8.21 % trisaccharide and 2.74 % tetrasaccharide were obtained. Regioselectivity was absolute for the 1→2 linkage.
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Suwasono, S., Rastall, R.A. A highly regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using specific 1,2-α-mannosidase from Aspergillus phoenicis . Biotechnol Lett 18, 851–856 (1996). https://doi.org/10.1007/BF00127901
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DOI: https://doi.org/10.1007/BF00127901