Summary
Living Kluyveromyces fragilis yeast cells were succesfully entrapped in calcium alginate gel beads at cell loadings of 4 to 16 g yeast (0.8 to 3.2 g d.m.) per 1 g of sodium alginate. In batch systems, about 90 % conversion in 48 h was obtained both with free and immobilized yeast using demineralized whey of 5 to 10 % lactose content as substrate. In continuous packed-bed column operation nearly a constant 2 % product ethanol concentration could be maintained at 5 % substrate lactose level for at least one month.
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References
Anon. (1980) Biosources Digest 2(8), 3.
Antonov, S. F., Kreshenin, P. F., Boganov, V. N., Khramtsov, A. G. & Eremin, C. E. (1977) Brit. Pat. 1,491,405.
Antonov, S. F., Kreshenin, P. F., Boganov, V. N., Khramtsov, A. G. & Eremin, C. E. (1978) U.S. Pat. 4,086,368.
Bernstein, S., Tzeng, C. H. & Sisson, D. (1977) Biotechnol. Bioeng. Symp. 7, 1–9.
Burgess, K. J. & Kelly, J. (1978) Irish J. Fd Sci. Technol. 3(1), 1–9.
Cunningham, M. L., Friend, B. A. & Shahani, K. M. (1975) J. Dairy Sci. 62, Suppl. 1, 60.
Friend, B. N. & Shahani, K. M. (1978) New Zealand J. Dairy Sco. Technol. 14, 149–152.
Gavel, J. & Kosikowski, F. V. (1978a) J. Fd Sci. 43, 1031–1032.
Gavel, J. & Kosikowski, F. V. (1978b) J. Fd Sci. 43, 1717–1718.
Hansen, R. C. (1980) North Eur. Dairy J. 46(1–2), 10–17.
Hesse, A. (1948) Z. Lebensm.-Unters. u. -Forsch. 89, 499–508.
Holsinger, V. H., Posati, L. P. & Devilloiss, E. D. (1978) J. Dairy Sci. 57, 849–859.
Hägerdal, B. (1980) Proc. 6th Int'l Fermentation Symp., 20.–25. July, 1980, London, Ontario, Canada. Pergamon Press, New York (in press).
Kolat, F. B. (1980) Process Biochem. 15(7), 2–8.
Kosikowski, F. V. & Wzorek, W. C. (1977) J. Dairy Sci. 60, 1982–1986.
Latham-Guillaume, M., Moulin, G. & Galzy, P. (1978) Lait 59, 489–498.
Linko, P. (1980a) In Food Process Engineering, Vol. 2, Enzyme Engineering in Food Processing (P. Linko & J. Larinkari, eds), Applied Science Publ., London, 27–39.
Linko, P. (1980b) Proc. 6th Int'l Fermentation Symp., 20.–25. July, 1980, London, Ontario, Canada. Pergamon Press, New York (in press).
Linko, P. (1981) Proc. Int'l Symp. Nutritive Sweeteners, 30. March –1. April, 1981, Weybridge, England. Applied Science Publ., London (in press).
Linko, Y.-Y. & Linko, P. (1981) Biotechnol. Lett. 3(1), 21–26.
Linko, Y.-Y. & Linko, P. (1980) In Food Process Engineering, Vol. 2, Enzyme Engineering in Food Processing (P. Linko & J. Larinkari, eds), Applied Svience Publ., London, 81–91.
Mann, J. (1980) Dairy Ind. Int'l 45(3), 47–48.
Mahmoud, M. K. & Kosikowski, F. V. (1978) J. Dairy Sci. 61, Suppl. 1, 114–115.
Moulin, G., Boza, H. & Galzy, P. (1980) Biotechnol. Bioeng. 22, 2375–2381.
O'Leary, V. S., Green, R., Sullivan, B. C. & Holsinger, V. H. (1977a) Biotechnol. Bioeng. 19, 1019–1035.
O'Leary, V. S., Sutton, C., Bencivengo, H., Sullivan, B. C. & Holsinger, V. H., (1977b) Biotechnol. Bioeng. 19, 1689–1702.
Philliskirk, G. & Yates, H. J. (1978) Brit. Pat. 1,504,618.
Poznanski, S., Leman, J., Bednarski, V., Smelich, V., Kowalewska, J., Chadkowski, M. & Wieliczka, R. (1978) Nahrung 22, 275–285.
Reesen, L. & Strube, R. (1978) Process Biochem. 13(11), 21–24.
Roland, J. F. & Alm, W. L. (1975) Biotechnol. Bioeng. 22, 1935–1948.
Wada, M., Kato, J. & Chibata, I. (1981) Eur. J. Appl. Microbio. Biotechnol. 11, 67–71.
Viller, R., Dillon, J. & Manderson, G. (1979) Sun 2, Proc. Int'l Sol. Energy Soc. Silver Jubilee Congr., 78–82.
Zoll, R. R., Kuipers, A., Muller, C. C. & Marshall, K. R. (1979) New Zealand J. Dairy Sci. Technol. 14, 79–83.
Zoll, R. R. (1980) Dairy Ind. Int'l 45(4), 30–39.
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Linko, YY., Jalanka, H. & Linko, P. Ethanol production from whey with immobilized living yeast. Biotechnol Lett 3, 263–268 (1981). https://doi.org/10.1007/BF00127392
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DOI: https://doi.org/10.1007/BF00127392