Summary
Isozyme variation in 25 accessions of wild and cultivated Vigna unguiculata, 49 accessions of seven wild species belonging to section Vigna, and 11 accessions of V. vexillata (subgenus Plectrotropis) was scored at 17 putative loci to assess genetic relationships within and among species. The wild species selected for this study are among those which carry important agronomical traits useful in cowpea (V. unguiculata) breeding programs. Allelic frequencies were calculated and Nei's genetic distances were obtained. Low levels of intraspecific variation were observed for V. heterophylla, V. luteola and V. racemosa, whereas the other species showed a higher polymorphism. Vigna unguiculata possessed intraspecific genetic distances comparable to those previously found by other authors. Most of the isozyme variation was apportioned among species. Although V. luteola and V. marina had an interspecific genetic distance resembling the range observed at intraspecific level, all the other species showed very high interspecific distances. Vigna unguiculata was relatively closer genetically to V. vexillata than to the species belonging to section Vigna.
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Abbreviations
- AUS:
-
Australia
- BDI:
-
Burundi
- BRA:
-
Brazil
- BWA:
-
Botswana
- CAF:
-
Central African Republic
- GHA:
-
Ghana
- CMR:
-
Cameroon
- COG:
-
Congo
- RI:
-
Costa Rica
- EGY:
-
Egypt
- ETE:
-
Ethiopia
- GAB:
-
Gabon
- GRC:
-
Greece
- ITA:
-
Italy
- KEN:
-
Kenya
- MOZ:
-
Mozambique
- NER:
-
Niger
- NGA:
-
Nigeria
- PAN:
-
Panama
- RWA:
-
Rwanda
- TCD:
-
Chad
- TZA:
-
Tanzania
- ZAF:
-
South Africa
- ZAR:
-
Zaire
- ZMB:
-
Zambia
References
Ahmad, F., P.M., Gaur & A.E., Slinkard, 1992. Isozyme polymorphism and phylogenetic interpretations in the genus Cicer L. Theor. Appl. Genet. 83: 620–627.
Bringhurst, R.S., S., Arulsekar, J.F., HancockJr. & V., Voth, 1981. Electrophoretic characterization of strawberry cultivars. J. Amer. Soc. Hort. Sci. 106: 684–687.
Cardy, B.J., C.W., Stuber & M.M., Goodman, 1980. Techniques for starch gel electrophoresis of enzymes from maize (Zea mays L.). Department of Statistics Mimeo Series, No. 1317, North Carolina State University, Raleigh, NC.
Chiang, H.S. & S.R., Singh, 1988. Pod hairs as a factor in Vigna vexillata resistance to the pod sucking bug. Entomologia Experimentalis et Applicata 47: 195–199.
FAO/IBPGR, 1973. Plant Genetic Resources Newsletter 49: 45–48.
Fatokun, C.A., 1991. Wide hybridization in cowpea; problems and prospects. Euphytica 54: 137–140.
Fatokun, C.A., D., Danesh, N.D., Young & E.L., Stewart, 1993. Molecular taxonomic relationships in the genus Vigna based on RFLP analysis. Theor. Appl. Genet. 86: 97–104.
Fatokun, C.A. & B.B., Singh, 1987. Interspecific hybridization between Vigna pubescens and V. unguiculata (L.) Walp. through embryo rescue. Plant Cell, Tissue and Organ Culture 9: 229–233.
Galasso, I., D., Pignone & P., Perrino, 1993. Cytotaxonomic studies in Vigna. II. Heterochromatin characterization in Vigna unguiculata and three related wild species. Caryologia 4: 275–282.
Gepts, P., V., Llaca, R.O., Nodari & L., Panella, 1992. Analysis of seed proteins, isozymes and RFLPs for genetic and evolutionary studies in Phaseolus. In: H.F., Linskens & J.F., Jackson (Eds.), pp. 63–93, Springer, Berlin Heidelberg, New York.
Gottlieb, L.D., 1981. Electrophoretic evidence and plant populations. In: L., Feinhold (Ed.) Progress in Phytochemistry, vol 7, pp. 1–46, Pergamon Press, New York.
International Institute of Tropical Agriculture, 1988. IITA Annual Report and Research Highlight 1978/1988, Ibadan, Nigeria.
Jaaska, V., 1994. Isoenzyme evidence on the systematics of Hordeum section Marina (Poaceae). Pl. Sys. Evol. 191: 213–226.
Jaaska, V., & V., Jaaska, 1988. Isoenzyme variation in the genera Phaseolus and Vigna (Fabaceae) in relation to their systematics: aspartate aminotransferase and superoxide dismutase. Pl. Syst. Evol. 159: 145–159.
Lu, J., & B., Pickersgill, 1993. Isozyme variation and species relationships in peanut and its wild relatives (Arachis L.-Leguminosae). Theor. Appl. Genet. 85: 550–560.
Maréchal, R., J.M., Mascherpa & F., Stainier, 1978. Etude taxonomique d'un groupe d'espèces des genres Phaseolus et Vigna (V. unguiculata) sur la base de données morphologiques et polliniqes, traitées pour l'analyse informatique. Boissiera 28: 1–273.
Mowrey, B.D. & D.J., Werner, 1990. Phylogenetic relationships among species of Prunus as inferred by isozyme markers. Theor. Appl. Genet. 80: 129–133.
Nei, M., 1972. Genetic distance between populations. Am. Nat. 106: 283–292.
Ng, N.Q., 1990. Recent developments in cowpea germplasm collection, conservation, evaluation, and reseearch at the Genetic Resources Unit, IITA. In: N.Q., Ng & L.M., Monti (Eds.), Cowpea Genetic Resources, pp. 13–28, IITA, Ibadan, Nigeria.
Ng, N.Q., 1995 (in press). Cowpea Vigna unguiculata (Leguminosae-Papilionideae). In: J., Smartt & N.W., Simmonds (Eds.), Evolution of crop plants, 2nd edition, Longman, England.
Ng, N.Q. & R., Maréchal, 1985. Cowpea taxonomy, origin and germplasm. In: S.R., Singh & R.O., Rachie (Eds.), Cowpea Research Production and Utilization, pp. 10–21, John Wiley & Sons, New York, NY.
Ng, N.Q. & S., Padulosi, 1991. Cowpea gene pool distribution and crop improvement. In: N.Q., Ng, P., Perrino, F., Attere & Z., Zedan (Eds.), Crop Genetic Resources of Africa, Vol. II, pp. 161–174, IITA, IBPGR, UNEP and CNR, The Trinity Press, UK.
Padulosi, S., 1993. Genetic diversity, taxonomy and ecogeographic survey of the wild relatives of cowpea (Vigna unguiculata (L.) Walp.). PhD Thesis, Faculty of Science, Université Catholique de Louvain-La Neuve, Belgique.
Padulosi, S. & N.Q., Ng, 1993. A useful and unexploited herb, Vigna marina (Leguminosae-Papilionideae) and the taxonomic revision of its genetic diversity. Bull. Jard. Bot. Nat. Belg. 62: 119–126.
Paino D'Urzo, M., M., Pedalino, S., Grillo, R., Rao & M., Tucci, 1990. Variability in major seed proteins in different Vigna species. In: N.Q., Ng & L.M., Monti (Eds.), Cowpea Genetic Resources, pp. 90–100, IITA, Ibadan, Nigeria.
Panella, L. & P., Gepts, 1992. Genetic relationships within Vigna unguiculata (L.) Walp. based on isozyme analyses. Genet. Res. Crop Evol. 39: 71–88.
Pasquet, R.S., 1993a. Variation at isozyme loci in wild Vigna unguiculata (Fabaceae, Phaseoleae). Pl. Syst. Evol. 186: 157–173.
Pasquet, R.S., 1993b. Classification infraspécifique des formes spontanées de Vigna unguiculata (L.) Walp. (Fabaceae) á partir des données morphologiques. Bull. Jard. Bot. Nat. Belg. 62: 127–173.
Pienaar, B.J., 1992. A taxonomic revision of the genus Vigna Savi (Fabaceae) in Southern Africa. Masters Thesis, Faculty of Science, University of Pretoria, South Africa.
Selander, R.K., M.H., Smith, S.Y., Yang, W.E., Johnson & J.B., Gentry, 1971. Biochemical polymorphism and systematics in the genus Peromyscus: I. Variation in the old field mouse (Peromyscus polionotus). Univ. Tex. Pub. 7103: 49–90.
Sneath, P.H.A. & R.R., Sokal, 1973. Numerical taxonomy. Freeman, San Francisco.
Sokal, R.R. & P.H.A., Sneath, 1963. Principles of numerical taxonomy, Freeman, San Francisco.
Vaillancourt, R.E., N.F., Weeden & J., Barnard, 1993. Isozyme diversity in the cowpea species complex. Crop Sc. 33: 606–613.
Vaillancourt, R.E. & N.F., Weeden, 1993. Lack of similarity between Vigna unguiculata and other species of subgenus Vigna (Leguminosae). Can. J. Bot. 71: 586–591.
Wendel, J.F. & N.F., Weeden, 1989. Visualization and interpretation of plant isozymes. In: D.E., Soltis & P.M., Soltis (Eds.), Isozymes in Plant Biology, pp. 5–45, Dioscorides Press, Portland, Oregon.
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Sonnante, G., Piergiovanni, A.R., Ng, Q.N. et al. Relationships of Vigna unguiculata (L.) Walp., V. vexillata (L.) A. Rich. and species of section Vigna based on isozyme variation. Genet Resour Crop Evol 43, 157–165 (1996). https://doi.org/10.1007/BF00126759
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DOI: https://doi.org/10.1007/BF00126759