Abstract
Characteristics of regenerated plants obtained from hairy roots (Ri-transformed plants) of Vinca minor L., a producer of a pharmaceutically important indole alkaloid, vincamine, were investigated. A previously established Ri-transformed clone, Vm-101, proliferates rapidly in vitro, displays a high degree of lateral branching and rapid shoot elongation and has a growth index 2.5 times that of an untransformed plant. The addition of 2.2 μM benzyladenine to the culture medium increased the shoot number but did not decrease the growth index. Vincamine content in the leaves of in vitro-cultured Vm-101 was twice that in the cultured untransformed plant. These results suggest that multiple shoot culture of Ri-transformed plants may be an excellent tool for in vitro vincamine production.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Garnier J, Kunesch N, Siou E, Poisson J, Kunesch G & Koch M (1975) Etude des cultures de tissus de Vinca minor isolement d'un lignane, le lirioresinol B. Phytochemistry 14: 1385–1387
Hagimori M, Matsumoto T & Obi Y (1982) Studies on production of Digitalis by plant tissue culture. Plant Physiol. 69: 653–656
Hirata K, Yamanaka A, Kurano N, Miyamoto K & Miura Y (1987) Production of indole alkaloids in multiple shoot culture of Catharanthus roseus (L). Don. Agr. Biol. Chem. 51: 1311–1317
Hirata K, Horiuchi M, Ando T, Miyamoto K & Miura Y (1990) Vindoline and catharanthine production in multiple shoot cultures of Catharanthus roseus. J. Ferment. Bioeng. 70: 193–195
Lui J H C & Staba E J (1979) Effect of precursors on serially propagated Digitalis lanata leaf and root cultures. Phytochemistry 18: 1913–1916
Matsumoto T & Tanaka N (1991) Production of phytoecdysteroids by hairy root cultures of Ajuga reptans var. atropurpurea. Agr. Biol. Chem. 55: 1019–1025
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Oniscu C, Macoveanu M, Horoba E, Cojocaru M & Potorac E (1985) The study of the extraction process of vincamine. Rev. Roumaine Chem. 30: 807–815
Petiard V & Demarly Y (1972) Mise en evidence de glucosides et d'alcaloides dans les cultures de tissue vegetaux. Ann. Amélior. Plantes 22: 361–374
Stapfer R E & Heuser C W (1985) In vitro propagation of periwinkle. Hort. Science 20: 141–142
Tanaka N & Matsumoto T (1993a) Characterization of Ajuga plant regenerated from hairy roots. Plant Tiss. Cult. Lett. 10: 78–83
Tanaka N & Matsumoto T (1993b) Regenerants from Ajuga hairy roots with high productivity of 20-hydroxyecdysone. Plant Cell Rep. 13: 87–90
Tanaka N, Takao M, Matsumoto T & Machida Y (1993) Transformation of Agrobacterium rhizogenes strain MAFF03–01724 by electroporation. Ann. Phytopath. Soc. Jpn. 59: 587–593
Tanaka N, Takao M & Matsumoto T (1994) Agrobacterium rhizogenes-mediated transformation and regeneration of Vinca minor L. Plant Tiss. Cult. Lett. 11: 191–198
Tanaka N & Oka A (1994) Restriction endonuclease map of the root-inducing plasmid (pRi1724) of Agrobacterium rhizogenes strain MAFF03–01724. Biosci. Biotech. Biochem. 58: 297–299
Tepfer D (1984) Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37: 959–967
Toivonen L (1993) Utilization of hairy root cultures for production of secondary metabolites. Biotechnol. Prog. 9: 12–20
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tanaka, N., Takao, M. & Matsumoto, T. Vincamine production in multiple shoot culture derived from hairy roots of Vinca minor . Plant Cell Tiss Organ Cult 41, 61–64 (1995). https://doi.org/10.1007/BF00124087
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00124087