Abstract
Ellagic acid, a plant phenol present in various foods consumed by humans, has been reported to have both anti-mutagenic and anti-carcinogenic potential. To evaluate the potential anti-carcinogenic property of ellagic acid, we tested its effects on the toxicity of ben-zo[a]pyrene and benzo[a]pyrene, 7,8-dihydrodiol and binding of benzo[a]yrene to DNA in cultured human bronchial epithelial cells. The toxicity of ellagic acid itself for human bronchial epithelial cells was also determined. Using a colony-forming efficiency assay, it was found that a nontoxic concentration of ellagic acid (5 μg/ml) enhanced the toxicity of benzo[a]pyrene.7,8-dihydrodiol in human bronchial epithelial cells. In contrast, ellagic acid at concentrations of l.5 and 3.0 μg/ml inhibited binding of benzo[a]pyrenemetabolites to DNA in these cells. An explanation for the potentiating effect of ellagic acid on the toxicity of benzo[a]pyrene, 7,8-dihydrodiol will require further investigation into the possible mechanisms of interaction between these two compounds.
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Abbreviations
- B[a]P:
-
benzo[a]pyrene
- B[a]P 7,8-DHD:
-
(±)trans-7,8-dihydro-7,8-dihydroxybenzo[a]pyrene
- B[a]PDE-1:
-
(±)-7β,8α-dihydroxy-9β,10β-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene
- B[a]PDE-2:
-
(±) 7β,8α-dihydroxy-9β,10β-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene
- B[a]PDE-1:dG:
-
N2-]10{7β,8β,9α-dihydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene]yl}:deoxyguanosine
- B[a]PDE-2:dG:
-
NZ-{10α-[7β,8α,9β-trihydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene]yl}:deoxyguanosine
- CFE:
-
colony forming efficiency
- EA:
-
ellagic acid
- HBE:
-
human bronchial epithelial
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Teel, R.W., Babcock, M.S., Dixit, R. et al. Ellagic acid toxicity and interaction with benzo[a]pyrene and benzo[a]pyrene 7,8-dihydrodiol in human bronchial epithelial cells. Cell Biol Toxicol 2, 53–62 (1986). https://doi.org/10.1007/BF00117707
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DOI: https://doi.org/10.1007/BF00117707