Abstract
The objective of this publication is to present a new dynamic aerobic biodegradation test method simulating a river. A laboratory cascade test system and standardized batch shake flask tests were used for biodegradation studies with the non-volatile and non-sorbing model compounds 2,4-dinitrophenol, naphthalene-1-sulphonic acid and sulphanilic acid. To be closer to the often very low concentrations of substances in the environment the concentrations of the compounds used were standard test concentrations and lower. 14C labelled compounds were measured at 50 μg/l, capillary electrophoresis at 5000 μg/l and the removal of dissolved organic carbon at 50000 μg/l. The test results obtained confirmed the known ultimate biodegradability of the test compounds and showed that biodegradation degrees, rates and degradation durations depended on the test systems, the concentrations of test compounds and the inocula. The river model is a suitable simulation test for natural dynamic surface waters which can be used to perform biodegradability studies at low test concentrations if adequate analytical tools, preferably radioactive-labelled substances, are available.
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Abbreviations
- BOD:
-
biochemical oxygen demand
- DAWT:
-
DOC-die-away test
- DNP:
-
2,4-dinitrophenol
- DOC:
-
dissolved organic carbon
- E:
-
effluent of laboratory wastewater treatment plants
- MOST:
-
modified OECD screening test
- NSA:
-
naphthalene-1-sulphonic acid
- P:
-
pond water
- SAA:
-
sulphanilic acid (=4-amino benzene sulphonic acid)
References
Alexander M (1981) Biodegradation of chemicals of environmental concern. Science 211: 132–138
Alexander M (1985) Biodegradation or organic chemicals. Environ. Sci. Technol. 18: 106–111
Alexander M (1994) Biodegradation and Bioremediation. Academic Press, San Diego
Bartholomew GW & Pfaender FW (1983) Influence of spatial and temporal variations on organic pollutant biodegradation rates in an estuarine environment. App. Environ. Microbiol. 45: 103–109
Boethling RS & Alexander M (1979a) Effect of concentration of organic chemicals on their biodegradation by natural microbial communities. Appl. Environ. Microbiol. 37: 1211–1216
Boethling RS & Alexander M (1979b) Microbial degradation of organic compounds at trace levels. Environ. Sci. Technol. 13: 989–991
Brilon C, Beckmann W & Knackmuss HJ (1981) Catabolism of naphthalenesulfonic acids by Pseudomonas sp. A3 and Pseudomonas sp. C22. Appl. Environ. Microbiol. 42: 44–55
Budack AM (1992) Einfluß der Inokulumskonzentration auf das Abbauergebnis in Testverfahren auf leichte biologische Abbaubarkeit gemäß OECD 301. Diploma thesis Technical University of Darmstadt, Germany
DIN — Deutsches Institut für Normung, Berlin — Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung (German standards on water quality) — DIN 38 406/5 Ammonium-Stickstoff; DIN 38 409/3 Gesamter organisch gebundener Kohlenstoff; DIN 38 409/51 Biochemischer Sauerstoffbedarf in n Tagen nach dem Verdünnungsprinzip; DIN 38 410/1 Allgemeine Hinweise, Planung und Durchführung von Fließgewässeruntersuchungen; DIN 38 410/2 Saprobienindex.
EEC — Directives, Annex to 92/69/EEC and 88/302/EEC. Official Journal of the European Communities L383 A and L133, Luxemburg (1988 and 1992)
Feigel BJ (1990) Synergistischer Abbau von 4-Aminobenzolsulfonat durch Hydrogenophaga palleronii und Agroberterium radiobacter. Thesis, University of Stuttgart
Feigel BJ & Knackmuss HJ (1993) Syntrophic interactions during degradation of 4-aminobenzenesulfonic acid by two species bacterial culture. Arch. Micorbiol. 159: 124–130
International Organization for Standardization (ISO), Geneva-Standard methods for the evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds — ISO 7827 Method by analysis of dissolved organic carbon (DOC); ISO 9408 Method by determining the oxygen demand in a closed respirometer; ISO 9439 Method by analysis of released carbon dioxide; ISO 11733 Activated sludge simulation test
Jannasch HW (1979) Microbial ecology of aquatic low habitats. In: Strategies of Microbial Life in Extreme Environments. Life Sciences Research Report 13: 243–260. Dahlem Konferenzen, Berlin
Johnson BT & Heitkamp MA (1984) Environmental and chemical factors influencing the biodegradation of phthalic acid esters in freshwater sediments. Environ. Poll. (Series B) 8: 101–118
Larson RL (1979) Estimation of biodegradation of potential of xenobiotic organic chemicals. Appl. Environ. Microbiol. 38: 1153–1161
Lenke H (1990) Mikrobieller Abbau von Nitrophenolen: 2,4-Dinitrophenole und 2,4,6.Trinitrophenol. Thesis, University of Stuttgart
Lenke H, Pieper DH, Bruhn C & Knackmuss H.J (1922) Degradation of 2,4-dinitrophenol by two Rhodococcus erythropolis strains, HL 24–1 and HL 24–2. Appl. Environ. Microbiol. 58:2928–2932
Locher HH, Thurnheer T, Leisinger T and Cook AM (1989) 3-Nitrobenzene-sulfonate, 3-Aminobenzenesulfonat, and 4-Aminobenzenesulfonat as sole carbon sources for bacteria. Appl. Environ. Microbiol. 55:492–494
Nesbitt HJ & Watson JR (1980) Degradation of the herbicide 2,4-D in river water I. Description of Study Area and Survy of Rate Determining Factors. Water Res. 14: 1683–1688 II. The role of suspended sediment, nutrients and water temperature. Water Res. 14: 1689–1694 (1980)
OECD — Guidelines for Testing of Chemicals of the Organisation for Economic Cooperation and Development, Paris (1993) — OECD 301A DOC Die-Away Test; OECD 301B CO2-Evolution Test; OECD 301E Modified OECD Screening Test; OECD 301F Manometric Respirometry Test; OECD 303A Aerobic Sewage Treatment Test.
Pagga U (1987) Biologischer Abbau von Stoffen bei geringen Konzentrationen. Z Wasser-Abwasser-Forsch. 20:101–107
Pagga U (1985) Stoffprüfungen in einem Kläranlagenmodell — Abbaubarkeits- und Toxizitätstests im BASF-Toximeter. Z.Wasser-Abwasser-Forsch. 18:222–232
Pfaender FK & Bartholomew GW (1982) Measurement of aquatic biodegradation rates by determining heterotrophic uptake of radiolabeled pollutants. Appl. Environ. Microbiol. 44: 159–164
Rubin HE, Subba-Rao RV & Alexander M (1982) Rates of mineralization of trace concentrations of aromatic compounds in lake water and sewage samples. Appl. Environ. Microbiol. 43: 1133–1138
Rubin HE & Alexander M (1984) Effect of nutrients of the rates of mineralization of trace concentrations of phenol and p-Nitrophenol. Environ. Sci. Technol. 17: 104–107
Schmidt SK, Alexander M & Shuler ML (1985) Predicting threshold concentrations of organic substrates for bacterial growth. J. theor. Biol. 114: 1–8
Seel H (1993) Frage nach der Übertragbarkeit von Abbauergebnissen aus standardisierten Methoden auf ein Flußmodell. Diploma thesis Fachhochschule für Technik und Wirtschaft Reutlingen, Germany
Scholz N & Müller FJ (1992) The Riverine Biocenosis Model (Aquatic Stair Case Model). Chemosphere 25: 563–579
Strotmann U, Schwarz H & Pagga U (1995) The CO2/DOC-combination test-a new method to determine the biodegradability of chemical compounds. Chemosphere 30: 525–538
Subba-Rao RV, Rubin HE & Alexander M (1982) Kinetics and extent of mineralisation of organic chemicals at trace levels in freshwater and sewage. Appl. Environ. Microbiol. 43: 1139–1150
Thomas JM, Yordy JR Amador JA & Alexander M (1986) Rates of dissolution and biodegradation of water-insoluble organic compounds. Appl.Environ.Microbiol. 52: 290–296
Wang YS, Subba-Rao RV & Alexander M (1984) Effects of substrate concentration and organic and inorganic compounds on the occurrence and rate of mineralization and cometabolism. Appl. Environ. Microbiol. 47: 1195–1200
Wiggins BA & Alexander M (1988). Role of chemical concentration and second carbon source in acclimation of microbial communities for biodegradation. Appl.Environ.Microbiol. 54:2803–2807
Zürrer D, Cook AM & Leisinger T (1987) Microbial desulfonation of substituted naphthalenesulfonic acids and benzenesulfonic acids. Appl. Environ. Microbiol. 53: 1459–1463
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Koziollek, P., Knackmuss, HJ., Taeger, K. et al. A dynamic river model for biodegradability studies. Biodegradation 7, 109–120 (1996). https://doi.org/10.1007/BF00114623
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DOI: https://doi.org/10.1007/BF00114623