Abstract
High anthocyanin-producing cell lines, which were grown in a dark or in a light-dark regime, were selected from callus cultures initiated from stem and leaf tissues of Aralia cordata Thunb. by small-cell-aggregate selection. To verify the optimum culture conditions for anthocyanin production, cells were tested by changing the various basal media, sucrose concentration and nitrogen source and concentration. Good growth was obtained in the dark on Linsmaier-Skoog's basal medium containing 1.0 mg l-1 2,4-d and 0.1 mg l-1 kinetin, 2% (w/v) sucrose and full strength of nitrogen concentration. However, the highest anthocyanin yield (10.3% dry wt) was obtained in the dark on B5 medium containing 1.0 mg l-1 2,4-d and 0.1 mg l-1 kinetin. Our results suggested that it has became feasible to find the most effective conditions for cell growth and anthocyanin production by optimizations of the nitrogen concentration and the ratio of NH4 + to NO3 - in the medium.
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Abbreviations
- B5:
-
Gamborg (Gamborg et al. 1968)
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- LS:
-
Linsmaier and Skoog (Linsmaier & Skoog 1965)
- MS:
-
Murashige and Skoog (Murashige & Skoog 1962)
- NN:
-
Nitsch and Nitsch (Nitsch & Nitsch 1967)
- WH:
-
White (White 1963)
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This paper is part 81 in the series ‘Studies on Plant Tissue Cultures’. For Part 80 see Furuya T, Sakamoto K, Iida K, Asada Y, Yoshikawa T, Sakai S & Aimi N (1992) Phytochemistry 31: 3065–3068.
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Sakamoto, K., Iida, K., Sawamura, K. et al. Anthocyanin production in cultured cells of Aralia cordata Thunb.. Plant Cell Tiss Organ Cult 36, 21–26 (1994). https://doi.org/10.1007/BF00048311
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DOI: https://doi.org/10.1007/BF00048311