Abstract
A method for plant regeneration of Iris via somatic embryogenesis is described. Root and leaf pieces from in vitro-grown plants of several genotypes of rhizomatous Iris sp. were cultured in vitro. Callus induction occurred only on root cultures incubated under low light intensity (35 μmol m-2 s-1) on two induction media containing 2,4-D (4.5 or 22.5 μM), NAA (5.4 μM) and kinetin (0.5 μM). Somatic embryos developed after transfer of callus onto four regeneration media containing 9 or 22 μM BA, or 5 μM kinetin and 2 μM TIBA or 9 μM BA and 4 μM TIBA. Plantlets could be obtained from these somatic embryos. Genotypic differences were found both in callus induction and somatic embryo formation, with I. pseudacorus responding better than I. versicolor or I. setosa. Cytological analysis performed on root tips of 80 regenerated plants revealed that two of the I. pseudacorus regenerants were tetraploid.
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Abbreviations
- 2,4-D:
-
dichlorophenoxy acetic acid
- NAA:
-
naphthaleneacetic acid
- BA:
-
6-benzyladenine
- TIBA:
-
2,3,5-triiodobenzoic acid
- IBA:
-
indolebutyric acid
References
Abe T & Futsuhara Y (1984) Varietal difference of plant regeneration from root callus tissues in rice. Japan. J. Breed. 34: 147–155
Abe T & Futsuhara Y (1985) Efficient plant regeneration by somatic embryogenesis from root callus tissues of rice (Oryza sativa L.). J. Plant Physiol. 121: 111–118
Ahuja MR (1983) Somatic cell differentiation and rapid clonal propagation of aspen. Silvae Genet. 32: 131–135
Berlyn CP & Miksche JP (1976) Botanical Microtechnique and Cytochemistry. Iowa State University Press, Ames (326 p)
Gerlach D (1977) Botanische Mikrotechnik: eine Einfürung Thieme, Stuttgard (311 p)
Gray DJ (1990) Somatic cell culture and embryogenesis in the Poaceae. In: Kasperbauer MJ (Ed) Biotechnology in Tall Fescue Improvement (pp 27–57). CRC Press. Boston
Hussey G (1975) Totipotency in tissue explants and callus of some members of the Liliaceae, Iridaceae and Amaryllidaceae. J. Exp. Bot. 26: 253–262
Kromer KD (1985) Regeneration of some monocotyledonous plants from subterranean organs in vitro. Acta Agrobotanica 38: 65–87
Kunitake H & Mii M (1990) Somatic embryogenesis and plant regeneration from protoplasts of asparagus (Asparagus officinalis L.). Plant Cell Rep. 8: 706–710
Lenz LW (1978) Iris classification. In: Warburton B (Ed) The World of Irises (pp 1–42). The American Iris Society, Wichita, Kansas.
Marousky F & West SH (1990) Somatic embryogenesis and plant regeneration from cultured mature caryopses of bahiagrass (Paspalum notatum Flugge). Plant Cell Tiss. Org. Cult. 20: 125–129
Meier MM, Fuchigami LH & Roberts AN (1975) Propagation of tall bearded irises by tissue culture. HortScience. 10: 479–480
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Ozias-Akins P & Vasil IK (1983) Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117: 40–44
Radojevic L, Sokic O, & Tucic B (1987) Somatic embryogenesis in tissue culture of Iris (Iris pumila L.). Acta Hort. 212: 719–723
Randolph LF & Cox LG (1943) Factors influencing the germination of Iris seed and the relation of inhibiting substances to embryo dormancy. Proc. Amer. Soc. Hort. Sci. 43: 284–300
Reuther G (1977) Embryoide differenzierungsmuster im kallus der Gattungen Iris und Asparagus. Ber. Deutsh. Bot. Ges. 90: 417–437
dosSantos AVP, Cutter E & Davey MR (1983) Origin and development of somatic embryos in Medicago sativa L. (Alfalfa). Protoplasma 117: 107–115
Stoltz LP (1977) Growth regulator effects on growth and development of excised mature Iris embryos in vitro. HortScience. 12: 495–497
Tisserat B (1985) Embryogenesis, organogenesis and plant regeneration. In: Dixon RA (Ed) Plant Cell Culture: A Practical Approach (pp 79–106). IRL Press, Oxford
van derLinde PCG, Hol GMGM, Blombarnhoorn GJ, vanAartrijk J & deKlerk GJ (1988a) In vitro propagation of Iris hollandica Tub. cv Prof. Blaauw. Regeneration on bulb-scale explants. Acta Hort. 226: 121–128
van derLinde PCG & Hol GMGM (1988b) Tissue culture of Iris. Propagation is much improved. Bloembollenculture 99: 36–37.
Wenzel G & Uhrig H (1981) Breeding for nematode and virus resistance in potato via anther culture. Theor. Appl. Gen. 59: 333–340
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Laublin, G., Saini, H.S. & Cappadocia, M. In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises. Plant Cell Tiss Organ Cult 27, 15–21 (1991). https://doi.org/10.1007/BF00048200
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DOI: https://doi.org/10.1007/BF00048200