Abstract
Mesophyll protoplasts of species of series Juglandifolia (Solanum rickii, S. lycopersicoides, S. ochranthum and S. juglandifolium) were isolated and cultured in liquid nutrient media TM-2 or KM8P. The cell colonies formed were transferred onto agar-solidified media TM-3 or GM, and 10 to 15 days later onto TM-4, PRM, MS3ZG, KK or C regeneration media. Formation of the shoots for S. rickii and S. lycopersicoides was observed after 30 to 35 days on regeneration medium. The regenerated shoots were rooted on hormone-free MS medium. Morphological and cytogenetic analyses have shown that somaclonal variants might arise in the course of plant regeneration from protoplasts of these species.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BAP:
-
6-benzylaminopurine
- 2, 4-D:
-
2, 4 dichlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige and Skoog medium
- B5:
-
Gamborg medium
- TRIS:
-
tris(hydroxymethyl)aminomethane
References
Brewer GJ (1970) An introduction to isozyme techniques. Academic Press, New York
Evans DA, Sharp WR & Medina-Filho HP (1984) Somaclonal and gametoclonal variation. Amer. J. Bot. 71: 759–774
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158
Gavrilenko TA & Piven NM (1990) The culture of mesophyll protoplasts of some diploid wild species of Solanum. Res. Bull. Vavilov Inst. Plant Industry 204: 38–42
Handley LW & Sink KC (1985a) Plant regeneration of protoplasts isolated from suspension cultures of Solanum lycopersicoides Plant Sci. 42: 201–207
Handley LW & Sink KC (1985b) Plant regeneration of Solanum lycopersicoides Dun. from stem explants, callus and suspension culture. Plant Cell Tiss. Org. Cult. 5: 129–138
Handley LW, Nickels RL, Cameron MW, Moore PP & Sink KC (1986) Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides. Theor. Appl. Genet. 71: 691–697
Hosaka K, Ogihara Y, Matsubayashi M & Tsunewaki K (1984) Phylogenetic relationship between the tuberous Solanum species as revealed by restriction endonuclease analysis of chloroplast DNA. Jpn. J. Genet. 59: 349–369
Kao KN & Michayluk MR (1975) Nutrient requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110
Koblitz H & Koblitz D (1982) Experiments on tissue culture in the genus Lycopersicon Miller. Shoot formation from protoplasts of tomato long-term cell cultures. Plant Cell Rep. 1: 147–150
Kut SA & Evans DA (1982) Plant regeneration from cultured leaf explants of eight wild tomato species and two related Solanum species. In Vitro 18: 593–598
Marticorena C & Quezada M (1977) Notas sobre Solanum L. Bol. Soc. Biol. de Conception 51: 153–157
Medgyesy P, Menczel L & Maliga P (1980) The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and isolation of their somatic hybrids. Mol. Gen. Genet. 179: 693–698
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
O'Connell MA & Hanson MR (1986) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solanum rickii Theor. Appl. Genet. 72: 59–65
Ramulu KS (1991) Genetic variation in in vitro cultures and regenerated plants in tomato and its implications. In: Kalloo G (ed) Genetic Improvement of Tomato. Monographs on Theoretical and Applied Genetics, Vol 14 (pp 259–276). Springer-Verlag, Berlin
Ratushnyak YI, Piven NM & Rudas VA (1989) Protoplast culture and plant regeneration in Lycium barbarum L. Plant Cell Tiss. Org. Cult. 17: 183–190
Rick CM (1979) Biosystematic studies in Lycopersicon and closely related species of Solanum. In: Hawkes JG, Lester RN & Skelding AD (eds) The Biology and Taxonomy of the Solanaceae (pp 49–85). Academic Press, London
Rick CM (1987) Genetic resources in Lycopersicon. In: Nevins DJ & Jones RA (eds) Tomato Biotechnology. Plant Biology, Vol 4 (pp 17–26). Alan R. Liss, Inc., New York
Rick CM, De Verna JW, Chetelat RT & Stevens MA (1986) Meiosis in sesquidiploid hybrids of Lycopersicon esculentum and Solanum lycopersicoides. Proc. Natl. Acad. Sci. USA 83: 3580–3583
Shahin EA (1985) Totipotency of tomato protoplasts. Theor. Appl. Genet. 69: 235–240
Shahin EA & Spivey R (1986) A single dominant gene for Fusaria wilt resistance in protoplast-derived tomato plants. Theor. Appl. Genet. 73: 164–169
Shepard JF & Totten RE (1977) Mesophyll cell protoplasts of potato. Plant Physiol. 60: 313–316
Shepard JF, Bidney D & Shahin E (1980) Potato protoplasts in crop improvement. Science 208: 17–24
Sidorov VA, Kuchko AA & Gleba YY (1984) Genetic modification of Solanun tuberosum through protoplast culture and fusion. In: Rohlich P & Bacsy E (eds) Tissue Culture and RES (pp 529–534). Academiai Kiado, Budapest
Sidorov VA, Kuchko AA, Gajduk PP & Gleba YY (1985) Somaclonal variations among plants obtained from potato protoclones. Proc. Akad. Sci. USSR 281: 704–707
Tan MMC (1987) Somatic hybridization and cybridization in some Solanaceae. Ph.D. Thesis, Free Univ. Amsterdam, The Netherlands
Tan MMC, Boerrigter HS & Kool AJ (1987) A rapid procedure for plant regeneration from protoplasts isolated from suspension cultures and leaf mesophyll cells of wild Solanum species and Lycopersicon pennellii. Plant Sci. 49: 63–72
Vorobjeva GA, Surikov IM, Gavrilenko TA & Pavlov AV (1992) Intergeneric hybridization in tomato Lycopersicon esculentum Mill. and Solanum lycopersicoides Dun. Bull. Appl. Bot. Genet. Plant Breed. (Russia) 148: 45–52
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kochevenko, A.S., Ratushnyak, Y.I. & Gleba, Y.Y. Protoplast culture and somaclonal variability of species of series Juglandifolia . Plant Cell Tiss Organ Cult 44, 103–110 (1996). https://doi.org/10.1007/BF00048186
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00048186