Abstract
Hypericum brasiliense, a non-domesticated plant has been shown to have useful medicinal properties. This plant has not been cultivated so a protocol for mass propagation based on selection of superior clones was developed and a protocol established for the culture of callus cells that could be used for in vitro metabolite production. A micropropagation method based on amplification of nodal buds was developed, by selection, from ten seedling clones that were examined for growth rate, multiplication rate and rooting. The effect of various basal media, growth regulator types and concentrations were examined for optimal callus induction. Optimal callus induction occured on either Murashige and Skoog's or Gamborg's media supplemented with 1 to 2 mg l−1 of 2,4-dichlorophenoxyacetic acid.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- B5:
-
Gamborg's medium
- 2,4-Dscd:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indolacetic acid
- MS:
-
Murashige & Skoog's medium
- NAA:
-
naphtaleneacetic acid
References
Bütter KB, Bütter B & Schaffner W (1994) In vitro propagation of Hypericum perforatum: impact of thidiazuron and 6-benzyloaminopurine. Abstracts of the VIII IAPTC Congress, Firenze, Italy (p. 62)
Cardona ML, Fernandez I, Pedro JR & Serrano (1990) Xanthones from Hypericum reflexum. Phytochemistry 29: 3003–3006
Cellárová E, Kimáková K & Brutovská R (1992a) Multiple shoot formation and phenotypic changes of R0 regenerants in Hypericum perforatum L. Acta Biotechnol 12: 445–452
Cellárová E, Kimáková K & Brutovská R (1992b) Multiple shoot formation in Hypericum perforatum L. and variability of R0. Biol Plant 34 (Suppl): 536
Decosterd LA, Stoecki-Evans H, Chapuis JC, Sorda B & Hostettmann K (1989) New cell-growth inhibitory cyclohexadienone derivatives from Hypericum calycinum. Helv. Chim. Acta 72: 1833–1845
Fehr MJ, Carpenter SL & Petrich JW (1994) The role of oxygen in the photoinduced antiviral activity of hypericin. Bioorgan. & Med. Chem. Let. 4: 1339–1344
Fehr MJ, McCloskey MA & Petrich JW (1995) Light induced acidification by the antiviral agent hypericin. J. of the Am. Chem. Soc. 117: 1833–1836
Fernandez ML & Alcaraz MJ (1991) Antiinflamatory activity and inhibition of arachidonic acid metabolism by flavoniods. Agents and Action 32: 283–288
Gai F, Fehr MJ & Petrich JW (1993) Ultrafast excited-state processes in the antiviral agent hypericin. J. of the Am. Chem. Soc. 115: 3384–3385
Gamborg OL, Miller PA & Ojima K (1986) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158
Grattapaglia D & Machado MA (1990) Micropropagação. In: Torres AC & Caldas LS (eds) Técnicas e Aplicações da Cultura de Tecidos de Plantas (pp 99–170). ABCTP/EMBRAPA-CNPH, Brasilia
Illg RD, (1990) Variação somaclonal. In: Torres AC & Caldas LS (eds) Técnicas e Aplicações da Cultura de Tecidos de Plantas (pp 287–297). ABCTP/EMBRAPA-CNPH, Brasília
Ishiguro K, Yamaki M, Kashihara M, Takagi S & Isoi K (1990) A chromene from Hypericum japonicum. Phytochemistry 29: 1010–1011
Jimenez CR (1980) Hipericáceas. In: Reitz R (ed) Flora Ilustrada Catarinesee (pp 1–33). CNPq/IBDF/HBR, Itajaí
Kaneda N, Pezzuto JM, Soejarto DD, Kingharn AD, Farnsworth NR, Santisuk T, Tuchinda P, Udchachon J & Reutrakul V (1991) Plant anticancer agents 4D. New cytotoxic flavonoids from Mutingia calobura roots. J. Nat. Prod. 54: 196–206
Kartnig T & Brantner A (1990) Secondary constituents in cell cultures of Hypericum perforatum and Hypericum maculatum. Planta Med. 56: 634
Liebes L, Mazur Y, Freeman D, Lavie D, Lavie G, Kudler N, Mendoza S, Levin B, Hoechster H & Mervelo D (1991) A method for the quantification of hypericin, an antiviral agent, in biological fluids by High Performance Liquid Chromatography. Anal Biochem 195: 77–85
Moraleda G, Wu TT, Jibert AR, Aldrich CE, Condreay LD, Larsen SH, Tang JC, Colarino JM & Masen WS (1993) Inhibition of duck hepatitis B virus replication by hypericin. Antiviral Res 20: 235–248
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nakanishi S, Kobayashi E, Nakano H, Suzuki K & Tamaoki T (1989) Hypericin and pseudohypericin specifically inhibit protein kinase c: possible relation to their antiretroviral activity. Biochem Biophys Res Communications 165: 1207–1213
Oganesyan ET, Saraf AF, Simonyan AV & Shiryaev IN (1991) Examining the structure activity relationship in the series of flavonoids 9. Allergenic activity of chalcones. Khimiko Farmatsevtichesskii Zhurnal 25: 18–21
Picq M, Cheav SL & Pringent AF (1991) Effect of two flavonoids compounds on central nervous systems. Analgesic activity. Life Sci 49: 1979–1988
Rocha LM (1991) Estudo quimico e farmacológico de Hypericum brasiliense Choisy. M.S. Dissertation NPPN/UFRJ, Rio de Janeiro
Saraf AS & Oganesyan ET (1991) Flavonoids as potential antiallergic agents-A review. Khimiko Farmatseutticheskii Zhurnal 25: 4–7
Seabra RM & Correia Alves A (1989) Mangiferin and chlorogenic acid from Hypericum species. Planta Med 4: 404–405
Seabra RM & Correia Alves A (1991) Quercetin-3′-sulphate from Hypericum elodes. Phytochemistry 30: 1344–1345
Serkedjieva J, Manolokova N, Zgonia K, Nowosielska I, Zawilinska B & Grzybek J (1990) Sarothralin G. a new antimicrobial compound from Hypericum japonicum. Planta Med 56: 274–276
Stevenson NR & Lenard J (1993) Antiretroviral activities of hypericin and rose bengal-Phodynamic effects on friend leukenia virus infection of mice. Antiviral Res 21: 119–128
Weber ND, Muray BK, North JA & Wood SG (1994) The antiviral agent hypericin has in vitro activity against HSV-1 through non-specific association with viral and cellular membranes. Antiviral Chemistry & Chemotherapy 5: 83–90
White PR (1943) A Handbook of Plant Tissue Culture. The Jackes Cattel Press, Lancaster
Yazaki K & Okuda T (1990) Procyadins in callus and multiple shoot cuhures of Hypericum erectum. Planta Med 56: 490–495
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cardoso, M.A., de Oliveira, D.E. Tissue culture of Hypericum brasiliense Choisy: Shoot multiplication and callus induction. Plant Cell Tiss Organ Cult 44, 91–94 (1996). https://doi.org/10.1007/BF00048184
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00048184