Abstract
Shoot regeneration was achieved from in vitro-produced leaves of Elaeagnus angustifolia L. Half-leaf explants from the terminal part of the shoot produced more shoots than explants from the basal part of the in vitro-derived shoots on agar-solidified WPM medium supplemented with 1 μM benzyladenine (BA). In liquid medium of the same formulation, compact shoots that did not elongate were formed on the explants. Leaf cross-section explants (1 mm thick) produced shoots both on solid and liquid medium with 1 μM BA, whereas again compact shoots were formed with 10 μM BA. Further shoot development on these explants was promoted by their transfer to fresh solid medium containing 1 μM BA and 1 μM gibberellic acid (GA3).
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Abbreviations
- BA:
-
benzyladenine
- GA3 :
-
gibberellic acid
- WPM:
-
woody plant medium
References
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Economou, A.S., Maloupa, E.M. Regeneration of Elaeagnus angustifolia from leaf segments of in vitro-derived shoots. Plant Cell Tiss Organ Cult 40, 285–288 (1995). https://doi.org/10.1007/BF00048135
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DOI: https://doi.org/10.1007/BF00048135