Abstract
Somatic embryos from immature cotyledons in peanut (Arachis hypogaea) were initiated on media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-d). Over 90% primary embryogenesis and 41–46% repetitive embryogenesis were obtained 12 weeks after initiation by maintaining embryogenic cultures on medium containing 20 mg 1-1 2,4-d. Maintenance of cultures on medium with 30 or 40 mg I-1 2,4-d resulted in lower primary and secondary embryogenesis, and proliferation of nonembryogenic callus. Transfer of embryogenic cultures to a secondary medium with 10 or 20 mg I-1 2,4-d significantly enhanced secondary embryogenesis compared to basal medium without the growth regulator. The use of Murashige & Skoog versus Finer's media had no significant effect on embryogenesis (85–95%), repetitive embryogenesis (11–37%) or mean embryo number. Secondary embryogenesis was also maintained for over one year by repeated subculture of isolated somatic embryos on medium with 20 mg I-1 2,4-d.
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Abbreviations
- B5:
-
Gamborg et al. medium (Gamborg et al. 1968)
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- FN:
-
Finer & Nagasawa medium (Finer & Nagasawa 1968)
- MS:
-
Murashige & Skoog medium (Murashige & Skoog 1962)
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Baker, C.M., Wetzstein, H.Y. Repetitive somatic embryogenesis in peanut cotyledon cultures by continual exposure to 2,4-d . Plant Cell Tiss Organ Cult 40, 249–254 (1995). https://doi.org/10.1007/BF00048131
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DOI: https://doi.org/10.1007/BF00048131