Abstract
Clones encoding β-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding β-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic β-1,3-glucanase showed 68% nucleotide homology to gnl cDNA. Northern blot analysis using the Hevea cDNA as probe detected a mRNA of 1.3 kb which was expressed at higher levels in latex than in leaf. In situ hybridization analysis using petiole sections from Hevea localized the β-1,3-glucanase mRNA to the laticifer cells. Genomic Southern analysis suggested the presence of a low-copy gene family encoding β-1,3-glucanases in H. brasiliensis.
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Chye, ML., Cheung, KY. β-1,3-Glucanase is highly-expressed in laticifers of Hevea brasiliensis . Plant Mol Biol 29, 397–402 (1995). https://doi.org/10.1007/BF00043663
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DOI: https://doi.org/10.1007/BF00043663