Abstract
Morphological, cytological, isozyme and chloroplast DNA analyses were used to determine possible mechanism(s) for the loss of glutamate oxaloacetate transaminase-4 (GOT-4) isozyme activity in a somatic hybrid. Plant 204-1, derived by cell fusion between tomato (Lycopersicon esculentum) andSolanum lycopersicoides, was characterized for bothGot-4 and acid phosphatase-2 (Aps-2), two isozyme loci which are closely linked (recombination 2.5 cM). This hybrid was determined to be chimeric for bothGot-4 andAps-2. TheS. lycopersicoides plant used to provide cells for the fusion was determined to be heterozygous for bothGot-4 andAps-2. Only oneS. lycopersicoides allelic form ofAps-2 andGot-4 was found in plant 204-1. This observation indicated that either the alternative copy of theS. lycopersicoides chromosome region encodingGot-4 andAps-2 is deleted or the entire chromosome is absent. Plant 204-1 was cytologically determined to be aneuploid with approximately 62 chromosomes. Sixty-two somatic hybrids of separate callus origin were analysed for GOT-4 and a high proportion (27%) lacked theS. lycopersicoides form ofGot-4. The loss of this allele and the linkedAps allele most likely occurred in the suspension culture ofS. lycopersicoides used to provide cells for fusion.
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Conkle MT, PD Hodgskiss, LB Nummally, SC Hunter (1982) Starch gel electrophoresis of conifer seeds: A laboratory manual. US For Ser Gen Tech Rep PSW-64, p. 18
Evans DA, WR Sharp, HP Medina-Filho (1984) Somaclonal and gametoclonal variation. Amer J Bot 71: 759–774
Fobes JF, CM Rick (1986) Aps, Est and Got phenotypes. Rep Tomato Genet Coop 26: 7–9
Handley LW, RL Nickels, MW Cameron, PP Moore, KC Sink (1986) Somatic hybrid plants betweenLycopersicon esculentum andSolanum lycopersicoides. Theor Appl Genet 71: 691–697
Hoffmann F, T Adachi (1981) ‘Arabidobrassica’: Chromosomal recombination and morphogenesis in asymmetric hybrid cells. Planta 153: 586–593
Huang AHC, KDF Lui, RJ Youle (1976) Organelle-specific isozymes of aspartate-α — ketoglutarate transaminase in spinach leaves. Plant Phys 58: 110–113
Laemmli V (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London) 227: 680–682
Maniatis T, EF Fritsch, J Sambrook (1982) Molecular Cloning. Cold Spring Harbor Laboratory, New York
Medino-Filho HP (1980) Linkage of Aps-1, Mi and other markers on chromosome 6. Rep Tomato Genet Coop 30: 26–28
Palmer JD (1982) Physical and gene mapping of chloroplast DNA fromAtriplex triangularis andCucumis sativa. Nucleic Acids Res 10: 1593–1605
Rick CM, JF Fobes (1977) Linkage relations of some isozymic loci. Rep Tomato Genet Coop 27: 22–24
Rick CM, JF Fobes (1974) Association of an allozyme with nematode resistance. Rep Tomato Genet Coop 24: 25
Scandalios JG (1969) Genetic control of multiple molecular forms of enzymes in plants: A review. Biochem Genet 3: 37–39
Tanksley SD (1979) Linkage, chromosomal association and expression of Adh-1 and Pgm-2 in tomato. Biochem Genet 17: 1159–1167
Tanksley SD, GD Kuehn (1985) Genetics, subcellular localization, and molecular characterization of 6-phosphogluconate dehydrogenase isozymes in tomato. Biochem Genet 23: 441–454
Tanksley SD, CM Rick (1980) Isozymic gene linkage map of the tomato: applications in genetics and breeding. Theor Appl Genet 57: 161–170
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Moore, P.P., Sink, K.C. Characterization of aLycopersicon esculentum xSolanum lycopersicoides somatic hybrid lacking a glutamate oxaloacetate transaminase isozyme. Plant Cell Tiss Organ Cult 13, 39–47 (1988). https://doi.org/10.1007/BF00043045
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DOI: https://doi.org/10.1007/BF00043045