Abstract
Adventitious shoots and plantlets were regenerated in vitro from floral stem explants of Crinum macowanii (Bak.) (bush lily). The length (age) of the floral stem as well as the orientation and position of the explant disc in the floral stem were the most important factors affecting shoot regeneration. The highest number of shoots were regenerated when immature floral stems of 70–100 mm were used as starting material, using the middle or basal parts of the stem, and orientating the discs with their proximal ends on the medium. Combinations of kinetin (4.65 μM) and either indoleacetic acid (0.57 μM) or naphthaleneacetic acid (0.54 μM), or a combination of benzyladenine (4.44 μM) and 2,4-dichlorophenoxyacetic acid (0.45 μM) resulted in the highest numbers of shoots being regenerated. Although a slight degree of callus formation was noticed on the cut-edges of the discs, shoot formation did not occur via callus, but directly from the floral stem epidermis. Unrooted shoots were rooted on MS-medium containing 0.17 M sucrose.
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Abbreviations
- BA:
-
benzyladenine
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- NAA:
-
naphthaleneacetic acid
- 2,4-d :
-
2,4 dichlorophenoxyacetic acid
References
Alderson PG, Rice RD & Wright NA (1983) The potential for propagating tulips through tissue culture. The Plant Propagator 29: 10–13
De Bruyn MH, Ferreira DI, Slabbert MM & Pretorius J (1992) In vitro propagation of Amaryllis belladonna. Plant Cell Tiss. Org. Cult. 31: 179–184
De Fossard RA (1985) Tissue culture propagation: state of art. Acta Hort. 166: 83–91
Hosoki T & Asahira T (1980) In vitro propagation of Narcissus. HortScience 15: 602–603
Hussey G (1975) Totipotency in tissue explants and callus of some members of the Liliaceae, Iridaceae and Amaryllidaceae. J. Exp. Bot. 26: 253–262
Hyndman SE, Hasegewa PM & Bressan RA (1983) The rale of sucrose and nitrogen in adventitious root formation on cultured rose shoots. Plant Cell Tiss. Org. Cult. 2: 229–238
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Pearson AE (1979) Effects of auxin and cytokinins on morphogenesis of three varieties of Episcia. In: Conger BV (ed) Cloning Agricultural Plants Via In Vitro Techniques (pp 5–40). CRC Press, Boca Raton, Florida
Pierik RLM & Steegmans HHM (1986) Adventitious plantlet regeneration from floral stem explants of Nerine bowdenii W Watts. Ned. J. Agr. Sci. 34: 217–223
Pierik RLM, Blokker JS, Dekker MWC De Does H, Kuip AC, Van der Made TA, Menten YMJ & De Vetten NCMH (1990) Micropropagation of Hippeastrum hybrids. Integration of in vitro techniques in ornamental plant breeding. In: de Jong J (ed) Integration of In Vitro Techniques in Ornamental Plant Breeding. Proceedings, Symposium. 10–14 November 1990 (pp 21–27). Eucarpia, Wageningen, Netherlands
Sass JE (1966) Botanical Microtechnique. Iowa State Univ. Press (183 p)
Slabbert MM, De Bruyn MH, Ferreira DI & Pretorius J (1993) Regeneration of bulblets from twin scales of Crinum macowanii in vitro. Plant Cell Tiss. Org. Cult. 33: 133–141
Taeb AG & Alderson PG (1987) Micropropagation of tulip: optimising shoot production from floral stem explants. Acta Hort. 212: 677–681
Takayama S & Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro — effect of various cultural conditions. Physiol. Plant. 46: 184–190
Wright NA & Alderson PG (1980) The growth of tulip tissues in vitro. Acta Hort. 109: 263–270
Ziv M, Halevy AH & Shilo R (1970) Organs and plantlets regeneration of Gladiolus through tissue culture. Ann. Bot. 34: 671–676
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Slabbert, M.M., de Bruyn, M.H., Ferreira, D.I. et al. Adventitious in vitro plantlet formation from immature floral stems of Crinum macowanii . Plant Cell Tiss Organ Cult 43, 51–57 (1995). https://doi.org/10.1007/BF00042671
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DOI: https://doi.org/10.1007/BF00042671