Abstract
Petioles, leaf discs and midribs of several olive (Olea europaea L.) cultivars, collected from potted greenhouse plants, field-grown and in vitro shoots, were used to test their morphogenic capacity. Adventitious shoots were induced only in petioles from in vitro-grown shoots of cultivars Moraiolo, Dolce Agogia and Halkidikis, grown on Olive Medium (OM) plus 18 μM zeatin within 4 to 5 weeks. Regeneration was achieved, both on Murashige and Skoog (MS) and on modified OM, only in the dark. The highest regeneration was achieved directly from the proximal part of the petioles after 2 to 3 weeks in media containing 5 to 40 μM thidiazuron, or with both 10 μM 2-isopentenyladenine +2.2 μM 6-benzyladenine with or without low auxin concentration (not more than 2.5 μM). A few adventitious shoots were also regenerated from callus when it was shifted from auxin and cytokinin media to cytokinin only medium. The regeneration potential was higher in petioles collected from apical nodes than from basal ones. The adventitious shoots were transferred to solid half-strength MS medium supplemented with 4.5 μM zeatin for further development. Several regenerated shoots were rooted and the plantlets hardened in the greenhouse. No apparent differences regarding morphological aspects were observed among the regenerated plantlets or with those obtained by stimulation of axillary buds.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BA:
-
6-benzyladenine
- IBA:
-
indole-3-butyric acid
- NAA:
-
1-naphthaleneacetic acid
- TDZ:
-
thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-ylurea)
- 2iP:
-
2-isopentenyladenine
- MS:
-
Murashige and Skoog medium
- 1/2 MS:
-
half strength MS
- OM:
-
Olive Medium
- BN:
-
Bourgin & Nitsch
References
Bao Z-H, Ma Y-F, Liu J-F, Wang K-J, Zhang P-F, Ni D-X & Yang W-Q (1980) Induction of plantlets from the hypocotyl of Olea europaea L. in vitro. Acta Bot. Sin. 2: 96–97 (in Chinese)
Bourgin J P & Nitsch J P (1967) Obtention de Nicotiana haploides à partir d'ètamines cultivèes in vitro. Ann. Physiol. Veg. 9: 377–382
Cañas L A & Benbadis A (1988) In vitro plant regeneration fromn cotyledon fragments of the olive tree (Olea europaea L.) Plant Sci. 54: 65–74
James D J (1987) Cell and tissue culture technology for the genetic manipulation of temperate fruit trees. Biotechnol. Genet. Eng. Rev. 5: 33–79
Mencuccini M & Corona C (1990) Genetic improvement of olive by in vitro culture. Proc. XXIII Int. Hort. Cong. Firenze, Aug. 27-Sept. 1, (p. 3126) (abstract)
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Rugini E (1984) In vitro propagation of some olive (Olea europaea L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Scientia Hort. 24: 123–134
Rugini E (1986) Olive. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry Trees, Vol 1 (pp 253–267). Springer-Verlag, Berlin, Heidelberg, New York, Tokyo
Rugini E (1988) Somatic embryogenesis and plant regeneration in Olive (Olea europaea L.). Plant Cell Tiss. Org. Cult. 14: 207–214
Rugini E & Fedeli E (1990) Olive (Olea europaea L.) as an oilseed crop. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry. Legumes and Oilssed I. (pp 593–641). Springer-Verlag, Berlin Heidelberg, New York, Tokyo
Scaramuzzi F & Roselli G (1986) Olive genetic improvement. Olea 17: 1–17
Stamp J A, Colby S M & Meredith C P (1990) Improved shoot organogenesis from leaves of grape. J. Amer. Soc. Hort. Sci. 115: 1038–1042
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mencuccini, M., Rugini, E. In vitro shoot regeneration from olive cultivar tissues. Plant Cell Tiss Organ Cult 32, 283–288 (1993). https://doi.org/10.1007/BF00042290
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00042290