Abstract
The heat shock (hs) response during plant growth and development was analyzed in tobacco and Arabidopsis using chimaeric β-glucuronidase reporter genes (hs-Gus) driven by a soybean hs promoter. Fluorimetric measurements and histochemical staining revealed high Gus activities in leaves, roots, and flowers exclusively after heat stress. The highest levels of heat-inducible expression were found in the vascular tissues. Without heat stress, a developmental induction of hs-Gus was indicated by the accumulation of high levels of Gus in transgenic tobacco seeds. There was no developmental induction of hs-Gus in Arabidopsis seeds. In situ hybridization to the RNA of the small heat shock protein gene Athsp17.6 in tissue sections revealed an expression in heat-shocked leaves but no expression in control leaves of Arabidopsis. However, a high level of constitutive expression of hs gene was detected in meristematic and provascular tissues of the Arabidopsis embryo. The developmental and tissue-specific regulation of the hs response is discussed.
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Abbreviations
- hs:
-
heat shock
- Hsp:
-
heat shock protein(s)
- hs:
-
Gus: heat-inducible Gus gene(s)
- HSE:
-
heat shock element(s)
- HSF:
-
heat shock factor
- X-gluc:
-
5-bromo-4-chloro-3-indolyl-β-D-glucuronide
- Gus:
-
β-glucuronidase
- DAF:
-
days after flowering
- SAR:
-
scaffold attachment region
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Prändl, R., Kloske, E. & Schöffl, F. Developmental regulation and tissue-specific differences of heat shock gene expression in transgenic tobacco and Arabidopsis plants. Plant Mol Biol 28, 73–82 (1995). https://doi.org/10.1007/BF00042039
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DOI: https://doi.org/10.1007/BF00042039