Abstract
A cDNA library from freshly isolated protoplasts was differentially screened using cDNAs from mesophyll cells, stressed leaf strips and cell suspension cultures. One of the selected clones, 6P229, turned out to encode a putative polypeptide showing homology to the btuE periplasmic protein of Escherichia coli and to animal selenium-dependent glutathione peroxidases. A major difference was that the putative selenocysteine in the active site was not encoded by the termination codon TGA. The 6P229 gene was found to be expressed in germinating seeds, in apex and in flowers, as well as in stressed tissues. This pattern of expression would be consistent with a key role in cellular metabolism such as defense against oxidative stresses.
Article PDF
Avoid common mistakes on your manuscript.
References
Akasaka M, Mizoguchi J, Yoshimura S, Watanebe K: Nucleotide sequence of a cDNA for rabbit glutathione peroxidase. Nucleic Acids Res 17: 2136 (1989).
Bowler C, Alliotte T, De Loose M, Van Montagu M, Inzé D: The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolid. EMBO J 8: 31–38 (1989).
Chambers I, Frampton J, Goldfarb P, Affara N, McBain W, Harrison PR: The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the ‘termination’ codon, TGA. EMBO J 5: 1221–1227 (1986).
Drotar A, Phelps P, Fall R: Evidence for glutathione peroxidase activities in cultured plant cells. Plant Sci 42: 35–40 (1985).
Friedrich MJ, De Veaux LC, Kadner RJ: Nucleotide sequence of the btuCED genes involved in vitamin B12 transport in Escherichia coli and homology with components of periplasmic-binding protein-dependent transport systems. J Bact 167: 928–934 (1986).
Ghyselinck NB, Dufaure JP: A mouse cDNA sequence for epididymal androgen-regulated proteins related to glutathione peroxidase. Nucl Acids Res 18: 7144 (1990).
Gunzler WA, Steffens GJ, Grossman A, Kim SMA, Otting F, Wendel A, Flohe L: The amino-acid sequence of bovine glutathione peroxidase. Hoppe-Seyler's Z Physiol Chem 365: 195–212 (1984).
Jamet E, Durr A, Fleck J: Absence of truncated genes in The amphidiploid Nicotiana tabacum. Gene 59: 213–221 (1987).
Jamet E, Parmentier Y, Durr A, Criqui M-C, Fleck J: Is ubiquitin involved in the dedifferentiation of higher plant cells? Cell Diff Dev 29: 37–46 (1990).
Lamattina L, Weil JH, Grienenberger JM: RNA editing at a splicing site of NADH dehydrogenase subunit IV gene transcript wheat mitochondria. FEBS Let 258: 79–83 (1989).
Mullenbach GT, Tabrizi A, Irvine BD, Bell GI, Hallewell RA: Sequence of a cDNA coding for human glutathione peroxidase confirms TGA encodes active site selenocysteine. Nucl Acids Res 15: 5484 (1987).
Overbaugh JM, Fall R: Characterization of a selenium-independent glutathione peroxidase from Euglena gracilis. Plant Physiol 77: 437–442 (1985).
Rioux C, Kadner RJ: Vitamin B12 transport in Escherichia coli K12 does not require the btuE gene of the btuCED operon. Mol Gen Genet 217: 301–308 (1989).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Van Regenmortel MHV: Tobamoviruses. In: Kurstak E (ed) Handbook of Plant Virus Infections and Comparative Diagnosis. Elsevier/North Holland Medical Press, Amsterdam (1981).
Yoshimura S, Takekoshi S, Watanebe K, Fujii-Kuriyama Y: Determination of nucleotide sequence of cDNA coding rat glutathione peroxidase. Nucl Acids Res 17: 2136 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Criqui, M.C., Jamet, E., Parmentier, Y. et al. Isolation and characterization of a plant cDNA showing homology to animal glutathione peroxidases. Plant Mol Biol 18, 623–627 (1992). https://doi.org/10.1007/BF00040684
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040684