Abstract
Cassava fertility and seed viability are frequently low, which can be a disadvantage in a breeding programme. An embryo culture method is described whereby embryonic axes are excised from mature seeds and placed on a culture medium containing 1.23 μM indolebutyric acid (IBA) at 30°C under continuous light. The number of plants recovered by embryo culture was much greater than the number recovered from conventional seed germination procedures.
Article PDF
Avoid common mistakes on your manuscript.
References
Capinpin JM and Bruce VC (1955) Floral biology and cytology of Manihot ultilissima. Phillippine Agric 39: 306–316
Collins GB and Grosser JW (1984) Culture of embryos. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Vol. 1. Laboratory procedures and their applications. New York: Academic Press pp 241–257
Jennings DL (1963) Variation in pollen and ovule fertility in varieties of cassava, and the effect of interspecific crossing on fertility. Euphytica 12: 69–76
Kartha KK, Gamborg OL, Constabel F and Shyluk JP (1974) Regeneration of cassava plants from apical meristems. Plant Sci Lett 2: 107–113
Martin F (1976) Cytogenetics and plant breeding of cassava; A review. Plant Breeding Abst 46: 909–916
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nartey F, Moller BL and Anderson MR (1974) Changes in the major constituents of Manihot esculenta seeds during germination and growth. Econ Bot 28: 145–154
Roca WM (1984) Cassava. In: Sharp WR, Evans DA, Ammirato PV and Yamada Y (eds) Handbook of plant cell culture Vol. 2. Crop species. New York: Macmillan pp 269–301
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Biggs, B.J., Smith, M.K. & Scott, K.J. The use of embryo culture for the recovery of plants from cassava (Manihot esculenta Crantz) seeds. Plant Cell Tiss Organ Cult 6, 229–234 (1986). https://doi.org/10.1007/BF00040008
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040008