Abstract
A method of using a buoyant polypropylene membrane floated on liquid medium to culture asparagus cells and protoplasts was examined. Compared with direct culture in lquid medium the method using a polypropylene membrane was found to be superior for small-volume culture of cells at low density as well as for the culture of protoplasts.
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Chin, CK., Kong, Y. & Pedersen, H. Culture of droplets containing asparagus cells and protoplasts on polypropylene membrane. Plant Cell Tiss Organ Cult 15, 59–65 (1988). https://doi.org/10.1007/BF00039889
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DOI: https://doi.org/10.1007/BF00039889