Abstract
Previous studies have shown that the development anomaly encountered in meristem culture, known as ‘vitreous plants’, is due to deficient lignin synthesis. This anomaly can be cured by addition of phloridzin to the culture medium. This study examines the activities of some enzymes involved in the synthesis of lignins and of flavonoids in normal and in vitreous plants of two apple cultivars. The results showed that all enzymes were consistently less active in the vitreous plants. This agrees with previous studies made on the hydroxycinnamate: CoA ligase activity in Prunus avium (L.) meristem-derived plants. The study on the substrate specificity of the enzyme demonstrates that while its activity is lower in the vitreous plant, its conformation is identical with that of the normal plant; the substrate that is specific to enzyme extracts of both sources is para-coumaric acid.
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Phan, C.T., Hegedus, P. Possible metabolic basis for the developmental anomaly observed in in vitro culture, called ‘vitreous plants’. Plant Cell Tiss Organ Cult 6, 83–94 (1986). https://doi.org/10.1007/BF00037761
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DOI: https://doi.org/10.1007/BF00037761