Abstract
An in vitro method for the establishment and storage of over 200 Saccharum spp. hybrid clones was developed that involved only 1 medium for shoot development and multiplication, and no decontamination procedures. Apical buds, from the leaf axils of developing leaves surrounding the apical meristem, were cultured on medium containing the plant growth regulators 6-benzylaminopurine (BAP) and 6-furfurylaminopurine (kinetin), and regenerated multiple shoots. Shoots transferred to medium containing naphthaleneacetic acid (NAA) developed roots. In vitro plants transferred to a medium containing half strength salts and vitamins without plant growth regulators were placed in storage at 18°C. After 12 months of storage plants were transferred to fresh medium and returned to storage. The genetic integrity of clones (based on phenotype assessment) was not affected by the in vitro culture method and up to 14 months of low-maintenance storage conditions. These in vitro plants will be further tested for genetic stability using biochemical and molecular techniques.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anon (1982) Aseptic Bud Cultures for Safer International Exchange of Sugar Cane Varieties-a Handbook of Methods. West Indies Central Sugar Cane Breeding Station (p. 11)
Grisham MP & Bourg D (1989) Efficiency of in vitro propagation of sugarcane plants by direct regeneration from leaf tissue and by shoot-tip culture. Amer. Soc. Sugar Cane Technol. 9: 97–102
Hendre RR, Mascarenhas AF, Nadgir AL, Pathak M & Jagannathan V (1975) Growth of mosaic virus-free sugarcane plants from apical meristems. Indian Phytopath. 28: 175–178
Hendre RR, Iyer RS, Kotwal M, Khuspe SS & Mascarenhas AF (1983) Rapid multiplication of sugarcane by tissue culture. Sugar Cane May/June: 5–8
Lee TSG (1987) Micropropagation of sugarcane (Saccharum spp.). Plant Cell Tiss. Org. Cult. 10: 47–55
Leifert C, Camotta H, Wright SM, Waites B, Cheyne VA & Waites WM (1991) Elimination of Lactobacillus plantarum, Corynebacterium spp., Staphylococcus saprophyticus and Pseudomonas paucimobilis from micropropagated Hemerocallis, Choisya and Delphinium cultures using antibiotics. J. Appl. Bact. 71: 307–330
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant. 15: 473–497
Paulet F, Acquaviva C, Eksomtramage T, Lu YH, D'Hont A & Glaszmann JC (1991) In vitro conservation of a sugar cane collection. Proc. 1st Meeting Assoc. Franç. Canne Sucr. 47–52
Sauvaire D & Galzy R (1978) Micro-propagation de la canne a sucre par bouturage in vitro action d'une auxine et d'une cytokinine. Agron. Trop. 36: 63–69
Scowcroft WR (1984) Genetic Variability in Tissue Culture. Impact on Germplasm Conservation and Utilisation. IBPGR Report (p. 41)
Sreenivasan TV & Sreenivasan J (1985) In vitro sugarcane germplasm storage. Sugar Cane Jan/Feb: 1–2
Wagih MRE (1989) The establishment and use of in vitro tissue culture techniques for the production of pathogen and Fiji disease virus-free clones of sugarcane. PhD Thesis, Department of Agriculture, The University of Queensland (p. 191)
Waterworth P & Kahn RB (1978) Thermotherapy and aseptic bud culture of sugarcane to facilitate the exchange of germplasm and passage through quarantine. Plant Dis. Rep. 62: 772–776
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taylor, P.W.J., Dukic, S. Development of an in vitro culture technique for conservation of Saccharum spp. hybrid germplasm. Plant Cell Tiss Organ Cult 34, 217–222 (1993). https://doi.org/10.1007/BF00036105
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00036105