Abstract
For the purpose of developing somatic embryogenesis in Prunus avium L., immature zygotic embryos, collected from five donor trees and sorted into two size classes (C1: 2.5–3.5 and C2: 3.6–4.5 mm), received various experimental treatments. When cultured for 10 days on an inductive medium containing 18.1 μM 2,4-dichlorophenoxyacetic acid (2,4-d) and 9.3 μM kinetin, then transferred to fresh medium without growth regulators, 2.5% of the C1 class cotyledons expressed direct somatic embryogenesis. C2 class cotyledons were less responsive. The response was also influenced by the chosen donor tree. In a few cases, spontaneous germination occurred. The presence of a root meristem was clearly demonstrated by histological examination of longitudinal sections. The replacement of half the amount of 2,4-d, present in the inductive medium mentioned above, by the same quantity of naphthaleneacetic acid reduced the incidence of somatic embryogenesis. Conversely, a rhizogenic response was strongly enhanced. When submitted to an inductive medium containing indoleacetic acid and zeatin without any subcultures for 3 months, C1 class cotyledons were the most morphogenic and developed leaves and cotyledon-like structures.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- NAA:
-
α-naphthaleneacetic acid
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De March, G., Grenier, E., Miannay, N. et al. Potential of somatic embryogenesis in Prunus avium immature zygotic embryos. Plant Cell Tiss Organ Cult 34, 209–215 (1993). https://doi.org/10.1007/BF00036104
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DOI: https://doi.org/10.1007/BF00036104