Abstract
The influence of several endogenous factors on micropropagation of mature elite trees of beech (Fagus sylvatica L.) has been investigated. February was the most beneficial month for explantation of dormant buds, since infection with endogenous bacteria was still low and in vitro growth of the plant material was the highest. The genotypes tested gave different results concerning their growth on various tissue culture media. Out of 51 mature grafted genotypes only seven could be established in vitro. Grafting of branches of mature stock plants on juvenile rootstocks resulted in a high increase of multiplication rate compared with corresponding mature material, which could not be subcultured in vitro. Apical buds, larger than 20 mm, from 1-year-old shoots proved to be the most suitable explant source. Plant material could be subcultured for several years and rooted successfully.
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Abbreviations
- BA:
-
benzyladenine
- BTM:
-
Broad-leaved Tree Medium
- GD:
-
Gresshoff Doy Medium
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige Skoog Medium
- PVP:
-
polyvinylpyrrolidone
- SH:
-
Schenk Hildebrandt Medium
- WPM:
-
Woody Plant Medium
References
Ahuja MR (1983) Somatic cell differentiation and rapid clonal propagation of aspen. Silvae Genet. 32: 131–135
Ahuja MR (1984 a) Isolation and culture of mesophyll protoplasts from mature beech trees. Silvae Genet. 33: 37–39
Ahuja MR (1984 b) In vitro induction of organogenesis in juvenile and mature beech. Silvae Genet. 33: 241–242
Bonga JM (1987) Clonal propagation of mature trees: problems and possible solutions. In: Bonga JM & Durzan DJ (Eds) Cell and Tissue Culture in Forestry, Vol 1 General Principles and Biotechnology (pp 249–271). Martinus Nijhoff Publ, Dordrecht
Chalupa V (1979) In vitro propagation of some broad-leaved forest trees. Commun. Inst. For. Cechosl. 11: 159–170
Chalupa V (1981) Clonal propagation of broad-leaved forest trees in vitro. Commun. Inst. Forest. Cechosl. 12: 255–271
Chalupa V (1985) In vitro propagation of Larix, Picea, Pinus, Quercus, Fagus, and other species using adenine-type cytokinins and thidiazuron. Commun. Inst. For. Cechosl. 14: 65–90
Franclet A (1991) Biotechnology in ‘rejuvenation’: hope for the micropropagation of difficult woody plants. Acta Hort. 289: 273–282
Franclet A, Boulay M, Bekkaoui F, Fouret Y, Verschoore-Martouzet B & Walker N (1987) Rejuvenation. In: Bonga JM & Durzan DJ (Eds) Cell and Tissue Culture in Forestry, Vol 1 General Principles and Biotechnology (pp 232–248). Martinus Nijhoff Publ, Dordrecht
Greenwood MS (1987) Rejuvenation of forest trees. In: Kossuth SV & Ross SD (Eds) Hormonal Control of Tree Growth (pp 1–12). Martinus Nijhoff Publ, Dordrecht
Gresshoff PM & Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107: 161–170
Joergensen J (1988) Embryogenesis in Quercus petraea and Fagus sylvatica. J. Plant Physiol. 132: 638–640
Joergensen J (1991) Androgenesis in Quercus petraea, Fagus sylvatica and Aesculus hippocastanum. In: Ahuja MR (Ed) Woody Plant Biotechnology (pp 353–354). Plenum Press, New York
Juncker B & Favre JM (1989) Clonal effects in propagating oak trees via in vitro culture. Plant Cell Tiss. Org. Cult. 19: 267–276
Lang H & Kohlenbach HW (1988) Callus formation from mesophyll protoplasts of Fagus sylvatica L. Plant Cell Rep. 7: 485–488
Lloyd G & McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb. Proc. Intl. Plant Prop. Soc. 30: 421–427
Meier-Dinkel A (1987) In vitro Vermehrung und Weiterkultur von Stieleiche (Quercus robur L.) und Traubeneiche (Quercus petraea (Matt.) Liebl.)). Allg. Forst-u.J.-Ztg 158: 199–204
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nadel BL, Altman A, Pleban S & Hüttermann A (1991 a) In vitro development of mature Fagus sylvatica L. buds. I. The effect of medium and plant growth regulators on bud growth and protein profiles. J. Plant Physiol. 138: 596–601
Nadel BL, Altman A, Pleban S, Kocks R & Hüttermann A (1991 b) In vitro development of mature Fagus sylvatica L. buds. II. Seasonal changes in the response to plant growth regulators. J. Plant Physiol. 138: 136–141
Pardos JA (1981) In vitro plants formation from stem pieces of Quercus suber L. In: AFOCEL (Ed) Colloque International sur la Culture ‘In Vitro’ des Essences Forestieres (pp 186–190). Fontainebleau, France
Pierik RLM (1990) Rejuvenation and micropropagation. Proceedings of the VIIth International Congress on Plant Tissue and Cell Culture (pp 91–101) Amsterdam
San José MC (1986) Influencia de la situation del explanto en la planta y del tamaño del tubo de cultivo en la multiplicaciõn in vitro de Quercus robur L. Phyton 46: 33–38
San José MC, Ballester A & Vieitez AM (1988) Factors affecting in vitro propagation of Quercus robur L. Tree Physiol. 4: 281–290
Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204
Vieitez AM, San-José MC & Vieitez E (1985) In vitro plantlet regeneration from juvenile and mature Quercus robur L. J. Hort. Sci. 60: 99–106
Vieitez FJ, Ballester A & Vieitez AM (1992) Somatic embryogenesis and plantlet regeneration from suspension cultures of Fagus sylvatica L. Plant Cell Rep. 11: 609–613
Volkaert H, Schoofs J, Pieters A & De Langhe E (1990) Influence of explant source on in vitro axillary shoot formation in oak seedlings. Tree Physiol. 6: 87–93
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Meier, K., Reuther, G. Factors controlling micropropagation of mature Fagus sylvatica . Plant Cell Tiss Organ Cult 39, 231–238 (1994). https://doi.org/10.1007/BF00035975
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DOI: https://doi.org/10.1007/BF00035975