Abstract
The effects of the tertiary amines tetracaine, brucine and dibucaine on photophosphorylation and control of photosynthetic electron transport in isolated chloroplasts of Spinacia oleracea were investigated. Tertiary amines inhibited photophosphorylation while the related electron transport decreased to the rates, observed under non-phosphorylating conditions. Light induced quenching of 9-aminoacridine fluorescence and uptake of 14C-labelled methylamine in the thylakoid lumen declined in parallel with photophosphorylation, indicating a decline of the transthylakoid proton gradient. In the presence of ionophoric uncouplers such as nigericin, no effect of tertiary amines on electron transport was seen in a range of concentration where photophosphorylation was inhibited. Under the influence of the tertiary amines tested, pH-dependent feed-back control of photosystem II, as indicated by energy-dependent quenching of chlorophyll fluorescence, was unaffected or even increased in a range of concentration where 9-aminoacridine fluorescence quenching and photophosphorylation were inhibited. The data are discussed with respect to a possible involvement of localized proton flow pathways in energy coupling and feed-back control of electron transport.
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Abbreviations
- 9-AA:
-
9-aminoacridine
- J e :
-
flux of photosynthetic electron transport
- PC:
-
photosynthetic control
- pH1 :
-
H+ concentration in the thylakoid lumen
- pmf:
-
proton motive force
- ΦP :
-
potential quantum yield of photochemistry of photosystem II (with open reaction centers)
- Q A :
-
primary quinone-type electron acceptor of photosystem II
- q Q :
-
photochemical quenching of chlorophyll fluorescence
- q E :
-
energy-dependent quenching of chlorophyll fluorescence
- q AA :
-
light-induced quenching of 9-amino-acridine fluorescence
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Laasch, H., Weis, E. Photosynthetic control, “energy-dependent” quenching of chlorophyll fluorescence and photophosphorylation under influence of tertiary amines. Photosynth Res 22, 137–146 (1989). https://doi.org/10.1007/BF00035444
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DOI: https://doi.org/10.1007/BF00035444