Abstract
Previous evidence has indicated that the 16S rRNA genes in certain species of Aeromonas may have a history of lateral transfer and recombination. A comparative analysis of patterns of 16S nucleotide sequence polymorphism among species of Rhizobium and Agrobacterium was conducted to determine if there is similar evidence for chimeric 16S genes in members of the Rhizobiaceae. Results from phylogenetic analyses and comparison of patterns of nucleotide sequence polymorphism in portions of rhizobial 16S genes revealed the same type of segment-dependent polymorphic site partitioning that was previously reported for Aeromonas. These results support the hypothesis that certain 16S segments in rhizobia may have a history of lateral transfer and recombination.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- 16S rRNA:
-
16S ribosomal ribonucleic acid
- 16S:
-
the 16S rRNA gene
References
Dams E, Hendriks L, Van de Peer Y, Neefs J-M, Smits G, Vandenbempt I and De Wachter R 1988 Compilation of small ribosomal subunit RNA sequences. Nucl. Acids Res. 16, r87-r173.
Eardly B D, Wang F-S, Whittam T S and Selander R K 1995 Species limits in Rhizobium populations that nodulate the common bean (Phaseolus vulgaris). Appl. Environ. Microbiol. 61, 507–512.
Graham P H, Sadowsky M J, Keyser H H, Barnet Y M, Bradley R S, Cooper J E, DeLey J, Jarvis B D W, Roslycky E B, Strijdom B W and Young J P W 1991 Proposed minimal standards for the description of new genera of species of root- and stem-nodulating bacteria. Int. J. Syst. Bacteriol. 41, 582–587.
Kumar S, Tamura K and Nei M 1993 MEGA: Molecular Evolutionary Genetic Analysis, version 1.01. The Pennsylvania State University, University Park, PA 16802, USA
Martinez-Murcia A J, Benlloch S and Collins M D 1992 Phylogenetic interrelationships of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int. J. Syst. Bacteriol. 42, 412–421.
Niebel H, Dorsch M and Stackebrandt E 1987 Cloning and expression in Escherichia coli of Proteus vulgaris genes for 16S ribosomal RNA. J. Gen. Microbiol. 133, 2401–2409.
Robison-Cox J F, Bateson M M and Ward D M 1995 Evaluation of nearest neighbor methods for detection of chimeric small-subunit rRNA sequences. Appl. Environ. Microbiol. 61, 1240–1245.
Saitou N and Nei M 1987 The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Sneath P H A 1993 Evidence from Aeromonas for genetic crossing-over in ribosomal sequences. Int. J. Syst. Bacteriol. 43, 626–629.
Stackebrandt E and Goebel E M 1994 Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44, 846–849.
Van Berkum P, Beyene D and Eardly B D 1996 Phylogenetic relationships among Rhizobium species nodulating the common bean (Phaseolus vulgaris L.) Int. J. Syst. Bacteriol. 46, 240–244.
Willems A and Collins M D 1993 Phylogenetic analysis of rhizobia and agrobacteria based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 43, 305–313.
Woese C R, Gutell R, Gupta R and Noller H F 1983 Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol. Rev. 47, 621–669.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eardly, B.D., Wang, F.S. & van Berkum, P. Corresponding 16S rRNA gene segments in Rhizobiaceae and Aeromonas yield discordant phylogenies. Plant Soil 186, 69–74 (1996). https://doi.org/10.1007/BF00035057
Issue Date:
DOI: https://doi.org/10.1007/BF00035057