Abstract
Genotypes representing the three botanical varieties of peanut (Arachis hypogaea L.) were assessed for somatic embryogenesis and subsequent plant conversion from mature zygotic embryo axes. Explants were initially cultured on Murashige and Skoog medium supplemented with 12.42 μM 4-amino-3,5,6-trichloropicolinic acid. Individual somatic embryos wer isolated from explant tissue and used to initiate repetitive liquid cultures. There were significant differences among genotypes and varieties for somatic embryo formation and plant regeneration using a single media sequence. Botanical variety fastigiata had a lower embryogenic frequency and produced significantly fewer embryos than either hypogaea or vulgaris, which were similar in response.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- EA:
-
zygotic embryo axes
- MS:
-
Murashige and Skoog (1962) medium
- picloram:
-
4-amino-3,5
- 6:
-
trichloropicolinic acid
References
Baker C M & Wetzstein H Y (1992) Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea. Plant Cell Rep. 11: 71–75
Boote K J (1982) Growth stages of peanut (Arachis hypogaea L.). Peanut Sci. 9: 35–40
Durham R E & Parrott W A (1992) Repetitive somatic embryogenesis from peanut cultures in liquid medium. Plant Cell Rep. 11: 122–125
Hazra S, Sathaye S S & Mascarenhas A F (1989) Direct somatic embryogenesis in peanut (Arachis hypogaea). Bio Technology 7: 949–951
McKently A H (1991) Direct somatic embryogenesis from axes of mature peanut embryos. In vitro Cell. Dev. Biol. 27P: 197–200
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Ozias-Akins P, Anderson W F & Holbrook C C (1992) Somatic embryogenesis in Arachis hypogaea L.: genotype comparison. Plant Sci. 83: 103–111
Parrott W A, Williams E G, Hildebrand D F & Collins G B (1989) Effect of genotype on somatic embryogenesis from immature cotyledons of soybean. Plant Cell Tiss. Org. Cult. 16: 15–21
Sellars R M, Southward G M & Phillips G C (1990) Adventitious somatic embryogenesis from cultured immature zygotic embryos of peanut and soybean. Crop Sci. 30: 408–414
Tian L N, Brown D C W, Voldeng H & Webb J (1994) In vitro response and pedigree analysis for somatic embryogenesis of long-day photoperiod adapted soybean. Plant Cell Tiss. Org. Cult. 36: 269–273
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McKently, A.H. Effect of genotype on somatic embryogenesis from axes of mature peanut embryos. Plant Cell Tiss Organ Cult 42, 251–254 (1995). https://doi.org/10.1007/BF00029995
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029995