Abstract
The homologous gene of the maize Vp1 gene was isolated from rice (Oryza sativa). Sequence analysis revealed that the rice Vp1 gene (Osvp1) encodes a protein of 728 amino acids and is interupted by 5 introns at positions identical to those of the maize gene. Osvp1 transcript was detected in developing embryo as early as 10 days after flowering and decreased toward maturity. Osvp1 transcript was also detected in dry as well as imbided mature embryos. The ability of Osvp1 gene product to activate a target gene was shown by transient expression experiments in rice suspension-cultured cell protoplasts using a reporter gene construct carrying the bacterial β-glucuronidase (GUS) gene fused to the promoter of OsEm gene, the rice homologue of the wheat Em gene.
Article PDF
Avoid common mistakes on your manuscript.
References
Chelsky D, Ralph R, Jonal G: Sequence requirements for synthetic peptide-mediated translocation to the nucleaus. Mol Cell Biol 9: 2487–2492 (1989).
Dennis ES, Sachs MM, Gerlach WL, Finnegan EJ, Peacock WJ: Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize. Nucl Acids Res 13: 727–743 (1985).
Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM: Isolation of the Arabidopsis AB13 gene by positional cloning. Plant Cell 4: 1251–1261 (1992).
Hattori T, Vasil V, Rosenkrans L, Hannah LC, McCarty DR, Vasil IK: The Viviparous-1 gene and abscisic acid activate the C1 regulatory gene for anthocyanin biosynthesis during seed maturation in maize. Genes Devel 6: 609–618 (1992).
Klosgen RB, Gierl A, Schwarz-Sommer Z, Saedler H: Molecular analysis of the waxy locus of maize. Mol Gen Genet 204: 237–244 (1986).
Koornneef M, Reuling G, Karssen CM: The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol Plant 61: 377–383 (1984).
Kyozuka J, Shimamoto K: Transformation and regeneration of rice protoplasts. In: Lindsey K (ed) Plant Tissue Culture Manual, pp. B1: 1–16. Kluwer Academic Publishers, Dordrecht (1991).
Litts JC, Erdman MB, Huang N, Karrer EE, Noueiry A, Quatrano RS, Rodoriguez RL: Nucleotide sequence of the rice (Oryza sativa) Em protein gene (Emp1). Plant Mol Biol 19: 335–337 (1992).
McCarty DR, Carson CB, Stinard PS, Robertson DS: Molecular analysis of viviparous-1: an abscisic acid insensitive mutant of maize. Plant Cell 1: 523–532 (1989).
McCarty DR, Hattori T, Carson CB, Vasil V, Vasil IK: The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell 66: 895–905 (1991).
Robertson DS: The genetics of vivipary in maize. Genetics 40: 745–760 (1955).
Robichaud CS, Wong J, Sussex IM: Control in vitro growth of viviparous embryo mutants of maize by abscisic acid. Devel Genet 1: 325–330 (1980).
Wang Z, Wu Z, Zheng F, Guo X, Zhang W, Hong M: Nucleotide sequence of the rice waxy gene. Nucl Acids Res 18: 5898 (1990).
Xie Y, Wu R: Molecular analysis of an alcohol dehydrogenase-encoding genomic clone (adh2) from rice. Gene 87: 185–191 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hattori, T., Terada, T. & Hamasuna, S.T. Sequence and functional analyses of the rice gene homologous to the maize Vp1 . Plant Mol Biol 24, 805–810 (1994). https://doi.org/10.1007/BF00029862
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029862