Summary
An approach to assess the extent of developmental gene expression of various regions of plastid (pt)DNA in mustard (Sinapis alba L.) is described. It involves cloning of most ptDNA regions. The cloned regions then serve as hybridization probes to detect and assess the abundance of complementary RNA sequences represented in total plastid RNA. By comparison of the hybridization pattern observed with plastid RNA from either dark-grown or light-grown plants it was found that many ptDNA regions are constitutively expressed, while several ‘inducible’ regions account for much higher transcript levels in the chloroplast than in the etioplast stage. The reverse situation, i.e. ‘repressed’ regions which would account for higher transcript levels in the etioplast, was not observed. The hybridization results obtained with RNA from ‘intermediatetype’ plastids suggest that transient gene expression is a common feature during light-induced chloroplast development. The time-course of gene expression differs for various ptDNA regions.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- kb:
-
kilobases
- ptDNA:
-
plastid DNA
References
Bedbrook, JR, Link, G, Coen, DM, Bogorad, L, Rich, A: Maize plastid gene expressed during photoregulated development. Proc Natl Acad Sci USA 75:3060–3064, 1978.
Bottomley, W, Smith, HJ, Bogorad, L: RNA polymerases of maize: partial purification and properties of the chloroplast enzyme. Proc Natl Acad Sci USA 68:2412–2416, 1971.
Ellis, RJ: Chloroplast proteins: Synthesis, transport, and assembly. Annu Rev Plant Physiol 32:111–137, 1981.
Gollmer, I, Apel, K: The phytochrome-controlled accumulation of mRNA sequences encoding the light-harvesting chlorophyll a/b protein of barley. Eur J Biochem 133:309–313, 1983.
Link, G, Chambers, SE, Thompson, JA, Falk, H: Size and physical organization of chloroplast DNA from mustard (Sinapis alba L.). Mol Gen Genet 181:454–457, 1981.
Link, G: Cloning and mapping of chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.). Nucleic Acids Res 9:3681–3694, 1981.
Link, G: Phytochrome control of plastid mRNA in mustard (Sinapis alba L.). Planta 154:81–86, 1982.
Mohr, H: Phytochrome and chloroplast development. Endeavour 1:107–114, 1977.
Parthier, B: The cooperation of nuclear and plastid genomes in plastid biogenesis and differentiation. Biochem Physiol Pflanzen 177:283–317, 1982.
Reisfeld, A, Gressel, J, Jakob, KM, Edelman, M: Characterization of the 32 000 dalton membrane protein I. Early synthesis during photoinduced plastid development of Spirodela. Photochem Photobiol 27:161–165, 1978.
Smith, SM, Ellis, RJ: Light-stimulated accumulation of transcripts of nuclear and chloroplast genes for ribulosebis-phosphate carboxylase. J Mol Appl Genet 1:127–137, 1981.
Southern, EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517, 1975.
Stiekema, WJ, Wimpee, CF, Silverthorne, J, Tobin, EM: Phytochrome control of the expression of two nuclear genes encoding chloroplast proteins in Lemna gibba L. G-3. Plant Physiol 72:717–724, 1983.
Link, G, Langridge, U: Structure of the chloroplast gene for the precursor of the Mr 32 000 photosystem II protein from mustard (Sinapis alba L.). Nucleic Acids Res 12:945–958, 1984.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Link, G. Hybridization study of developmental plastid gene expression in mustard (Sinapsis alba L.) with cloned probes for most plastid DNA regions. Plant Mol Biol 3, 243–248 (1984). https://doi.org/10.1007/BF00029660
Issue Date:
DOI: https://doi.org/10.1007/BF00029660