Abstract
We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tissue-specific and positively affected by light. Response times, both to dark and light conditions, are remarkably rapid.
A chimeric gene consisting of a 1.2 kb Fd promoter fragment fused to the β-glucuronidase reporter gene was transferred to tobacco. This fusion gene is expressed in a tissue-specific way; it shows high levels of expression in green leaves, as compared to root tissue.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bradford MM: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254 (1976).
Dente L, Cesareni G, Cortese R: pEMBL: a new family of single stranded-plasmids. Nucl Acids Res 11: 1645–1655 (1983).
Dobres MS, Elliott RC, Watson JC, Thompson WF: A phytochrome regulated pea transcript encodes ferredoxin I. Plant Mol Biol 8: 53–59 (1987).
Dutton JE, Rogers LJ, Haslett BG, Takruri IAH, Gleavens JT, Boulter D: Comparative studies on the properties of two ferredoxins from Pisum sativum L. J Exp Bot 31: 379–391 (1980).
Feinberg AP, Vogelstein B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).
Gidoni D, Brosio P, Bond-Nutter D, JBedbrook, PDunsmuir: Novel cis-acting elements in Petunia Cab gene promoters. Mol Gen Genet 215: 337–344 (1989).
Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnik PA, Cashmore AR: An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci USA 85: 7089–7093 (1988).
Hall DO, Rao KK: Ferredoxin. Encyclopedia of Plant Physiology 5: 206–216 (1977).
Hoekema A, vanHaaren M, Fellinger A, Hooykaas P, Schilperoort R: Non-oncogenic plant vectors for use in the Agrobacterium binary system. Plant Mol Biol 5; 85–89 (1985).
Horsch R, Fry J, Hoffman N, Eichholtz D, Rogers S, Fraley R: A simple and general method for transferring genes into plants. Science 227: 1229–1231 (1985).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3908 (1987).
Kaufman LS, Briggs WR, Thompson WF: Phytochrome control of specific mRNA levels in developing pea buds. The presence of both very low fluence and low fluence responses. Plant Physiol 78: 388–393 (1985).
Kaufman LS, Roberts LL, Briggs WR, Thompson WF: Phytochrome control of specific mRNA levels in developing pea buds. Kinetics of accumulation, reciprocity, and escape kinetics of the low fluence response. Plant Physiol 81: 1033–1038 (1986).
Kuhlemeier C, Fluhr R, Chua N-H: Upstream sequences determine the difference in transcript abundance of pea rbcS genes. Mol Gen Genet 212: 405–411 (1988).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Mitra A, An G: Three distinct regulatory elements comprise the upstream promoter region of the nopaline synthase gene. Mol Gen Genet 215: 294–299 (1989).
Murashige T, Skoog F: A revise medium for rapid growth and bio-assay with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Murray HG, Thompson WF: Rapid isolation of high molecular weight plant DNA. Nucl Acid Res 8: 4321–4325 (1980).
Robinson C, Ellis RJ: Transport of proteins into chloroplasts; partial purification of a chloroplast protease involved in the processing of imported precursor polypeptides. Eur J Biochem 142: 337–342 (1984).
Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe JH: Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143: 161–178 (1980).
Schulze-Lefert P, Dangl JL, Becker-Andre MB, Hahlbrock K, Schulz W: Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene. EMBO J 8: 651–656 (1988).
Smeekens S, vanBinsbergen J, Weisbeek P: The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone. Nucl Acids Res 13: 3179–3194 (1985).
Smeekens S, Bauerle C, Hageman J, Keegstra K, Weisbeek P: The role of the transit peptide in the routing of precursors toward different chloroplast compartments. Cell 46: 365–375 (1986).
Smeekens S, vanSteeg H, Bauerle C, Bettenbroek H, Keegstra K, Weisbeek P: Import into chloroplasts of a yeast mitochondrial protein directed by ferredoxin and plastocyanin transit peptides. Plant Mol Biol 9: 377–388 (1987).
Takahashi Y, Hase T, Wada K, Matsubara H: Ferredoxins in developing spinach cotyledons: the presence of two molecular species. Plant Cell Physiol 24: 189–198 (1983).
Takahashi Y, Mitsui A, Hase T, Matsubara H: Formation of the iron-sulfur cluster of ferredoxin in isolated chloroplasts. Proc Natl Acad Sci USA 83: 2434–2437 (1986).
Vorst O, Oosterhoff-Teertstra R, Vankan P, Smeekens S, Weisbeek P: Plastocyanin of Arabidopsis thaliana; isolation and characterization of the gene and chloroplast import of the precursor protein. Gene 65: 59–69 (1988).
Wedel N, Bartling D, Herrmann RG: Analysis of cDNA clones encoding the entire ferredoxin I precursor polypeptide from spinach. Bot Acta 101: 295–300 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vorst, O., van Dam, F., Oosterhoff-Teertstra, R. et al. Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants. Plant Mol Biol 14, 491–499 (1990). https://doi.org/10.1007/BF00027495
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00027495