Abstract
The production of resting eggs by the rotifer Brachionus plicatilis was tested at four salinities (9, 18, 27 and 36\%) and six concentrations of the alga Chlorella stigmatophora (0.25, 0.5, 1.0, 2.0, 4.0 and 6.0 × 106 cells ml−1). The results indicated that resting eggs were produced only at two salinities (9\% and 18\%) and that their number was affected by the amount of food provided. A model consisting of two generalized linear sub-models was built to evaluate the contribution of each of the tested food concentrations at the two salinities. The sub-models were used to distinguish between two different components of resting egg production: one related to the presence or absence of resting egg production, and the other to the number of resting eggs produced, given that production had occurred. Besides indicating the best combination of salinity and food concentration for obtaining large numbers of resting eggs, they revealed the contribution of internal population factors that were not controlled in the course of the experiment. The model identified the positive contribution of the relative number of females to males, and the negative association between high rotifer densities and the production of resting eggs. The results of the present study help in defining the optimal conditions for mass production of resting eggs, which are of potential importance in aquaculture.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Dixon, W. J. (ed.), 1983. BMOP statistical software. University of California Press, Los Angeles, CA, 734 pp.
Fukusho, K., 1983. Present status and problems in the culture of the rotifer for Brachionus plicatilis for fry production of marine fishes in Japan. In: R. Hector, (ed.), Symposium Internacional de Aquacultura, Coquimbo, Chile: 361–374.
Haberman, S., 1982. The analysis of dispersion of multinominal respose. J. am. Stat. Assoc. 77: 568–580.
Hagiwara, A., A. Hino & R. Hirano, 1988a. Effect of temperature and chlorinity on resting egg formation in the rotifer Brachionus plicatilis. Nippon Suisan Gakkaishi 54: 569–575.
Hagiwara, A., A. Hino & R. Hirano, 1988b. Comparison of resting egg formation among five Japanese stocks of the rotifer Brachionus plicatilis. Nippon Suidan Gakkaishi 54: 577–580.
Hagiwara, A., C. S. Lee, G. Miyamoto & A. Hino, 1989. Resting egg formation and hatching of the S-type Brachionus plicatilis rotifer at varying salinities. Mar. Biol. 103: 327–332.
Hino, A. & R. Hirano, 1977. Ecological studies on the mechanism of bisexual reproduction in the rotifer Brachionus plicatilis — II. Effects of cumulative parthenogenetic generation on the frequency of bisexual reproduction. Bull. Jap. Soc. Sci. Fish. 43: 1147–1155.
Hino, A. & R. Hirano, 1985. Relationship between the temperature given at the time of fertilized egg formation and bisexual reproduction patterns in the deriving strain of the rotifer Brachionus plicatilis. Bull. Jap. Soc. Sci. Fish. 51: 511–514.
Hino, A. & R. Hirano, 1988. Relationship between water chlorinity and bisexual reproduction rate in the rotifer Brachionus plicatilis. Nippon Suisan Gakkaishi 54: 1329–1332.
Hirata, H., 1980. Culture methods of the marine rotifer Brachionus plicatilis. Min. Rev. Data File Fish. Res. 1: 27–46.
Hosmer, D. W. & S. Lemeshaw, 1989. Applied Logistic Regressions. John Wiley & Sons Inc., USA. 307 pp.
Lubzens, E., 1981. Rotifer resting eggs and their application to marine aquaculture. Spec. Publ. Europ. Maricult. 6: 163–179.
Lubzens, E., 1989. Possible use of rotifer resting eggs and preserved live rotifers in Aquaculture. In N. De Pauw, E. Jaspers, H. Ackefors & N. Wilkins (eds), Aquaculture—A Biotechnology in Progress. Europ. Aquacult. Soc. Bredene, Belgium: 741–750.
Lubzens, E. & G. Minkoff, 1988. Influence of the age of algae fed to rotifers (Brachionus plicatilis O. F. Muller) on the expression of mixis in their progenies. Oecologia (Berlin) 75: 430–435.
Lubzens, E., R. Fishler & V. Berdugo-White, 1980. Induction of sexual reproduction in the rotifer Brachionus plicatilis reared in sea water. In H. J. Dumont & J. Green (eds), Rotatoria. Developments in Hydrobiology I. Dr W. Junk Publishers, The Hague: 55–58. Reprinted from Hydrobiologia 73.
Lubzens, E., G. Minkoff & S. Marom, 1985. Salinity dependence of sexual and asexual reproduction in the rotifer Brachionus plicatilis. Mar. Biol. 85: 123–126.
McCullogh, P. & J. A. Nelder, 1983. Generalized Linear Models. Chapman and Hall, London, 511 pp.
Minkoff, G., E. Lubzens & D. Kahan, 1983. Environmental factors affecting hatching of rotifer (Brachionus plicatilis) resting eggs. In B. Pejler, R. Starkweather & Th. Nogrady (eds), Biology of Rotifers. Developments in Hydrobiology 14. Dr W. Junk Publishers, The Hague: 61–69. Reprinted from Hydrobiologia 104.
Okauchi, M. & K. Fukusho, 1985. Different modes in carrying resting eggs between two wild types of the rotifer Brachionus plicatilis from Matsusaka. Nippon Suisan Gakkaishi 51: 1907.
Pourriot, R. & T. W. Snell, 1983. Resting eggs in rotifers. In B. Pejler, R. Starkweather & Th. Nogrady (eds), Biology of Rotifers. Developments in Hydrobiology 14. Dr W. Junk Publishers, The Hague: 213–224. Reprinted from Hydrobiologia 104.
Ruttner-Kolisko, A. 1972. Plankton rotifers, biology and taxonomy. Die Binnengewasser (suppl.) 26: 1–146.
Ruttner-Kolisko, A., 1983. The significance of mating processes for the genetics and for the formation of resting eggs in monogonont rotifers. In B. Pejler, R. Starkweather & Th. Nogrady (eds), Biology of Rotifers. Developments in Hydrobiology 14. Dr W. Junk Publishers, The Hague: 181–190. Reprinted from Hydrobiologia 104.
Snell, T. W., 1986. Effect of temperature, salinity and food level on sexual and asexual reproduction in Brachionus plicatilis (Rotifer). Mar. Biol. 92: 157–162.
Snell, T. W., 1987. Sex, population dynamics and resting egg production in rotifers. Hydrobiologia 144: 105–111.
Snell, T. W. & E. M. Boyer, 1988. Thresholds for mictic female production in the rotifer Brachionus plicatilis (Muller). J. exp. mar. Biol. Ecol. 124: 73–85.
Snell, T. W. & B. L. Garman, 1986. Encounter probabilities between male and female rotifers. J. exp. mar. Ecol. 97: 221–230.
Snell, T. W. & F. H. Hoff, 1986. The effect of environmental factors on resting egg production in rotifer Brachionus plicatilis. J. World Maricult. Soc. 16: 484–497.
Author information
Authors and Affiliations
Additional information
Deceased, September 1991.
Rights and permissions
About this article
Cite this article
Lubzens, E., Wax, Y., Minkoff, G. et al. A model evaluating the contribution of environmental factors to the production of resting eggs in the rotifer Brachionus plicatilis . Hydrobiologia 255, 127–138 (1993). https://doi.org/10.1007/BF00025830
Issue Date:
DOI: https://doi.org/10.1007/BF00025830