Summary
Lily cv. Harmony was inoculated with several Agrobacterium strains to study its susceptibility to Agrobacterium infection and transformation. Tumorous tissue formation on inoculated stem internodes of sterile-grown plantlets, as well as expression of a β-glucuronidase marker gene interrupted by an intron in cells of inoculated stem nodes, indicate that the monocotyledon Lilium is a host for Agrobacterium.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
De Cleene M., 1985. The susceptibility of monocotyledons to Agrobacterium tumefaciens. Phytopath. Z. 113: 81–89.
Dommisse E.M., D.W.M. Leung, M.L. Shaw & A.J. Conner, 1990. Onion is a monocotyledonous host for Agrobacterium. Plant Sci. 69: 249–257.
Dower W.J., J.F. Miller & C.W. Ragsdale, 1988. High efficiency transformation of E. coli by high voltage electroporation. Nuc. Acids Res. 16: 6127–6145.
Hemalsteens J-P., L. Thia-Toong, J. Schell & M.Van Montagu, 1984. an Agrobacterium transformed cell culture from the monocot Asparagus officinalis. EMBO J. 3: 3039–3041.
Hooykaas-Van Slogteren G.M.S., P.J.J. Hooykaas & R.A. Schilperoort, 1984. Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium. Nature 311: 763–764.
Jefferson R.A., 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405.
Koncz C. & J. Schell, 1986. The promoter of T1-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a noveltype of Agrobacterium binary vector. Mol. Gen. Genet. 204: 383–396.
Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.
Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press.
Van Aartrijk J. & G.J. Blom-Bamhoorn, 1983. Adventitious bud formation from bulb scale explants of Lilium speciosum Thunb. in vitro. Effects of wounding, TIBA and temperature. Z. Pflanzenphysiol. 110: 355–363.
Vancanneyt G., R. Schmidt, A. O'Connor-Sanchez, L. Willmitzer & M. Rocha-Rosa, 1990. Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium mediated plant transformation. Mol. Gen. Genet. 220: 245–250.
Usami S., S. Okamoto, I. Takebe & Y. Machida, 1988. Factor inducing Agrobacterium tumefaciens vir gene expression is present in monocotyledonous plants. Proc. Natl. Acad. Sci. USA 85: 3748–3752.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Langeveld, S.A., Gerrits, M.M., Derks, A.F.L.M. et al. Transformation of lily by Agrobacterium . Euphytica 85, 97–100 (1995). https://doi.org/10.1007/BF00023935
Issue Date:
DOI: https://doi.org/10.1007/BF00023935