Abstract
The TA regions of biotype III octopine/cucumopine (OC) Ti plasmids are closely related to the TL region of the biotype I octopine Ti plasmids pTiAch5 and pTi15955. Sequence analysis shows that the limited and wide host range biotype III OC TA regions are derived from a common ancestor structure which lacked the 6a gene found in the biotype I octopine TL region. The TA region of the wide host range OC Ti plasmids has conserved most of the original TL-like structure. In most wide host range OC isolates the TA-iaaH gene is inactivated by the insertion of an IS866 element. However, the TA region of the wide host range isolate Hm1 carries an intact TA-iaaH gene. This gene encodes a biologically active product, as shown by root induction tests and indole-3-acetic acid measurements.
The limited host range OC Ti plasmids pTiAB3 and pTiAg57 have shorter TA regions which are derived from a wide host range TA region. The AB3 type arose by an IS868-mediated, internal TA region deletion which removed the iaa genes and part of the ipt gene and left a copy of IS868 at the position of the deleted fragment. The pTiAB3 iaa/ipt deletion was followed by insertion of a second IS element, IS869, immediately 3′ of the ipt gene. pTiAg57 underwent the same iaa-ipt deletion as pTiAB3, but lacks the IS868 and IS869 elements.
Analysis of the various TA region structures provides a detailed insight into the evolution of the biotype III OC strains.
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References
Barker RF, Idler KB, Thompson DV, Kemp JD: Nucleotide sequence of the T-region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol Biol 2: 335–350 (1983).
Beaty JS, Powell GK, Lica L, Regier DA, Macdonald EMS, Hommes NG, Morris RO: Tzs, a nopaline Ti plasmid gene from Agrobacterium tumefaciens associated with trans-zeatin biosynthesis. Mol Gen Genet 203: 274–280 (1986).
Bonnard G, Tinland B, Paulus F, Szegedi E, Otten L: Nucleotide sequence, evolutionary origin and biological role of a rearranged cytokinin gene from a wide host range biotype III Agrobacterium strain. Mol Gen Genet 216: 428–438 (1989).
Bonnard G, Vincent F, Otten L: Sequence and distribution of IS866, a novel T-region-associated insertion sequence from Agrobacterium tumefaciens. Plasmid 22: 70–81 (1989).
Buchanan-Wollaston V, Passiatore JE, Cannon F: The mob and oriT mobilization functions of a bacterial plasmid promote its transfer to plants. Nature 328: 172–175 (1987).
De Meirsman C, Desair J, Vanderleyden J, Van Gol J, Jen CG: Similarities between nucleotide sequences of insertion elements of Agrobacterium tumefaciens and Pseudomonas savastanoi in relation to Agrobacterium tumefaciens TC-DNA. Nucl Acids Res 15: 10591 (1987).
De Meirsman C, van Soom C, Verreth C, Van Gol J, Vanderleyden J: Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37. Plasmid, in press.
Devereux J, Haeberli P, Marquess P: The program manual for the sequence analysis software package of the Genetics Computer Group. Nucl Acids Res 12: 387–395 (1987).
Dhaese P, De Greve H, Decraemer H, Schell J, van Montagu M: Rapid mapping of transposon insertion and deletion mutations in the large Ti plasmids of Agrobacterium tumefaciens. Nucl Acids Res 7: 1837–1849 (1979).
Gielen J, de Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M, van Montagu M, Schell J: The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5. EMBO J 3: 835–846 (1984).
Hoekema A, de Pater BS, Fellinger AJ, Hooykaas PJJ, Schilperoort RA: The limited host range of an Agrobacterium tumefaciens strain extended by a cytokinin gene from a wide host range. EMBO J 3: 3043–3047 (1984).
Hooykaas PJJ, Schilperoort RA: The molecular genetics of crown gall tumorigenesis. Adv Genet 22: 209–283 (1984).
Huss B, Bonnard G, Otten L: Isolation and functional analysis of a set of auxin genes with low root-inducing activity from an Agrobacterium tumefaciens biotype III strain. Plant Mol Biol 12: 271–283 (1989).
Huss B, Tinland B, Paulus F, Walter B, Otten L. Functional analysis of a complex oncogene arrangement in biotype III Agrobacterium tumefaciens strains. Plant Mol Biol 14: 173–186 (1990).
Kares C, Prinsen E, Van Onckelen H, Otten L: IAA synthesis and root induction with iaa genes under heat shock promoter control. Plant Mol Biol 15: 225–236 (1990).
Knauf VC, Panagopoulos CG, Nester EW: Genetic factors controlling the host range of Agrobacterium tumefaciens. Phytopathology 72: 1545–1549 (1982).
Knauf VC, Yanofsky M, Montoya A, Nester EW: Physical and functional map of an Agrobacterium tumefaciens plasmid that confers a narrow host range. J Bact 160: 564–568 (1984).
Machida Y, Sakurai M, Kiyokawa S, Ubuzawa A, Suzuki Y, Ikeda JE: Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues. Proc Natl Acad Sci USA 81: 7495–7499 (1984).
Messens E, Lenaerts A, Van Montagu M, Hedges RW: Genetic basis for opine secretion from crown gall tumour cells. Mol Gen Genet 199: 344–348 (1985).
Morris RO: Genes specifying auxin and cytokinin biosynthesis in phytopathogens. Ann Rev Plant Physiol 37: 509–538 (1986).
Nester EW, Gordon MP, Amasino RM, Yanofsky MF: Crown gall: a molecular and physiological analysis. Ann Rev Plant Physiol 35: 387–413 (1984).
Paulus F, Huss B, Bonnard G, Ridé M, Szegedi E, Tempé J, Petit A, Otten L. Molecular systematics of Agrobacterium tumefaciens biotype III Ti plasmids. Mol Plant-Microbe Interact 2: 64–74 (1989).
Paulus F, Ridé M, Otten L: Distribution of two Agrobacterium tumefaciens insertion elements in natural isolates: evidence for stable association between Ti plasmids and their bacterial hosts. Mol Gen Genet 219: 145–452 (1989).
Powell GK, Morris RO: Nucleotide sequence and expression of a Pseudomonas savastanoi cytokinin gene: homology with Agrobacterium tumefaciens tmr and tzs loci. Nucl Acids Res 14: 2555–2565 (1986).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A laboratory Manual, 2nd ed Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Schröder G, Waffenschmidt S, Weiler EW, Schröder J: The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur J Biochem 138: 387–391 (1984).
Szegedi E: Host range and specific (L+) tartrate utilization of biotype 3 of Agrobacterium tumefaciens. Acta Phytopath Acad Scient Hung 20: 17–22 (1985).
Thomashow MF, Knauf VC, Nester EW: Relationship between the limited and wide host range octopine-type Ti plasmids of Agrobacterium tumefaciens. J Bact 146: 484–493 (1981).
Thomashow LS, Reeves S, Thomashow MF: Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid. Proc Natl Acad Sci USA 8: 5071–5075 (1984).
Vanderleyden J, Desair J, De Meirsman C, Michiel K, Van Gol AP, Chilton M-D, Jen GC: Nucleotide sequence of an insertion sequence (IS) element identified in the T-DNA region of a spontaneous variant of the Ti plasmid pTiT37. Nucl Acids Res 14: 6699–6708 (1986).
Van Haute E, Joos H, Maes M, Warren E, van Montagu M, Schell J: Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of Ti plasmids of Agrobacterium tumefaciens. EMBO J 2: 411–417 (1983).
Ward JE, Akiyoshi DE, Regier D, Datta A, Gordon MP, Nester EW: Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid. J Biol Chem 263: 5804–5814 (1988).
Winans SC, Ebert PR, Stachel SE, Gordon MP, Nester EW: A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci. Proc Natl Acad Sci USA 83: 8278–8282 (1986).
Yamada T, Palm CJ, Brooks B, Kosuge T: Nucleotide sequence of the Pseudomonas savastanoi indoleacetic acid genes show homology with Agrobacterium tumefaciens T-DNA. Proc Natl Acad Sci USA 82: 6522–6526 (1985).
Yanofsky M, Lowe B, Montoya A, Rubin R, Krul W, Gordon MP, Nester EW: Molecular and genetic factors controlling host range in Agrobacterium tumefaciens. Mol Gen Genet 201: 237–246 (1985).
Yanofsky M, Montoya M, Knauf VC, Lowe B, Gordon MP, Nester EW: Limited-host-range plasmid of Agrobacterium tumefaciens: molecular and genetic analysis of transferred DNA. J Bact 163: 341–348 (1985).
Zambryski P, Tempé J, Schell J: Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants. Cell 56: 193–201 (1989).
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Paulus, F., Canaday, J., Vincent, F. et al. Sequence of the iaa and ipt region of different Agrobacterium tumefaciens biotype III octopine strains: reconstruction of octopine Ti plasmid evolution. Plant Mol Biol 16, 601–614 (1991). https://doi.org/10.1007/BF00023425
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DOI: https://doi.org/10.1007/BF00023425