Summary
Crossability and cytology were examined in F1, F2, B1 and hybridsplants of F1 hybrids of Brassica campestris and three wild relatives of B. oleracea, B. bourgeaui, B. cretica and B. montana, respectively. The F2 plants were obtained after self-and open pollination of the F1 hybrids. The B1 and hybrid plants were produced after the F1 hybrids backcrosses with B. campestris and crossed with B. napus, respectively. After crossing the F1 hybrids, many seeds of the F2, B1 and hybrid plants were harvested. Multivalent formation was high in the chromsome configuration for the PMCs of F2, B1 and hybrid plants, suggesting that crossing over might occur between them. Many different types of aneuploids were obtained in the progenies of the F2, B1 and hybrid plants. It is suggested that different types of normal egg cells may be produced by one-by-one or little-by-little chromosome addition. The possibility is discussed of gene transfer from B. bourgeaui, B. cretica and B. montana, to cultivated plants, B. campestris and B. napus.
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Attia, T. & G.R öbbelen, 1986. Cytogenetic relationship within cultivated Brassica analyzed in amphihaploids from the three diploid ancestors. Can. J. Genet. Cytol. 28: 323–329.
Bajaj, Y.P.S., 1990. Wide hybridization in legumes and oilseed crops through embryo, ovule, and ovary culture. In: Y.P.S. Bajaj (Ed), Biotechnology in agriculture and forestry, vol. 10. Legumes and oilseed crops I, pp. 1–37. Springer-Verlag, Berlin.
Gustafsson, M., 1982. Germplasm conservation of wild (n<9) Mediterranean Brassica species. Sveriges Utsadesforenings Tidskrift 92: 133–142.
Gustafsson, M., C.G ómez-Campo & A. Zamenis, 1983. Report from the first Brassica germplasm exploration in Greece 1982. Sveriges Utsadesforenings Tidskrift 93: 151–160.
Gustafsson, M. & S. Snogerup, 1983. A new subspecies of Brassica cretica from Peloponnisos, Greece. Bot. Chron. 3: 7–11.
Hosoda, T., 1961. Studies on the breeding of new types of Napus crops by means of artificial synthesis in genomes of genus Brassica. Memo. Tokyo Kyoiku Univ. 7: 1–94.
Inomato, N., 1977. Production of interspecific hybrids between Brassica campestris and Brassica oleracea by culture in vitro of excised ovaries. I. Effects of yeast extract and casein hydrolysate on the development of excised ovaries. Jpn. J. Breed. 27: 295–304.
Inomata, N., 1978. Production of interspecific hybrids in Brassica campestris x B. oleracea by culture in vitro of excised ovaries. I. Development of excised ovaries in the cross of various cultivars. Jpn. J. Genet. 53: 161–173.
Inomata, N., 1980. Hybrid progenies of the cross, Brassica campestris x B. oleracea. I. Cytogenetical studies on F1 hybrids. Jpn. J. Genet. 55: 189–202.
Inomata, N., 1983. Hybrid progenies of the cross, Brassica campestris x B. oleracea. II. Crossing ability of F1 hybrids and their progenies. Jpn. J. Genet. 58: 433–449.
Inomata, N., 1985a. Hybrid progenies of the cross, Brassica campestris x B. oleracea. III. Cytogenetical studies on F2, B1 and other hybrids. Jpn. J. Genet. 60: 359–371.
Inomata, N., 1985b. A revised medium for in vitro culture of Brassica ovaries. In: G.P. Chapman, S.H. Mantell & R.W. Daniels (Eds), Experimental manipulation of ovule tissues, pp. 164–176. Longmans, London.
Inomata, N., 1985c. Interspecific hybrids between Brassica campestris and B. cretica by ovary culture in vitro. Cruciferae Newsletter 10: 92–93.
Inomata, N., 1986. Interspecific hybrids between Brassica campestris and B. bourgeaui by ovary culture in vitro. Cruciferae Newsletter 11: 14–15.
Inomata, N., 1987. Interspecific hybrids between Brassica campestris and B. montana by ovary culture in vitro. Cruciferae Newsletter 12: 8–9.
Inomata, N., 1990. Interspecific hybridization in Brassica through ovary culture. In: Y.P.S. Bajaj (Ed), Biotechnology in agriculture and forestry, vol. 10. Legumes and oilseed crops I, pp. 367–384. Springer-Verlag, Berlin.
Inomata, N., 1991. Hybrid progenies of the cross, Brassica campestris x B. oleracea. IV. Crossability of F2, B1 and hybrid plants, and their progenies. Jpn. J. Genet. 66: 449–460.
Inomata, N., 1993. Embryo rescue techniques for wide hybridization. In: K.S. Labana, S.S. Banga, S.K. Banga (Eds), Breeding oilseed Brassicas, pp. 94–107. Springer-Verlag, Berlin.
Lee, K.H. & H. Namai, 1992. Pollen fertility and seed set percentage after backcrossing of sesquidiploids (AAC genomes) derived from interspecific hybrid between Brassica campestris L. (AA) and B. oleracea L. (CC) and frequency distribution of aneuploids in the progenies. Jpn. J. Breed. 42: 43–53.
Matsuzawa, Y., 1978. Studies on the interspecific hybridization in genus Brassica. I. Effects of temperature on the development of hybrid embryos and the inprovement of crossability by ovary culture in interspecific cross, B. campestris x B. oleracea. Jpn. J. Breed. 28: 186–196.
Mizushima, U., 1952. Karyo-genetical studies on Brassiceae. p. 112. Gihodo, Tokyo.
Nwankiti, O., 1971. Cytogenetics and breeding studies with Brassica. II. Progenies from backcrosses involving primary hybrids between B. napus and B. campestris. Hereditas 68: 35–46.
Pelletier, G., 1990. Cybrids in oilseed Brassica crops through protoplast fusion. In: Y.P.S. Bajaj (Ed), Biotechnology in agriculture and forestry, vol. 10. Legumes and oilseed crops I, pp. 418–433. Springer-Verlag, Berlin.
Sarashima, M., 1964. Studies on the breeding of artificially synthesized rape (Brassica napus). I. F1 hybrids between B. campestris group and B. oleracea group and the derived F2 plants. Japan. J. Breed. 14: 226–237.
Snogerup, S., 1980. The wild forms of the brassica oleracea group (2n<18) and their possible relations to the cultivated ones. In: S. Tsunoda, K. Hinata & C. Gómez-Campo (Eds), Brassica crops and wild allies, pp. 121–132. Jpn. Sci. Soc. Press, Tokyo.
Snogerup, S., M.Gustafsson & R.v.Bothmer, 1990. Brassica sect. Brassica (Brassicaceae). I. Taxonomy and variation. Willdenowia 19: 271–365.
Vambing, K. & K. Glimelius, 1990. Regeneration of plants from protoplasts of oilseed brassica crops. In: Y.P.S. Bajaj (Ed), Biotechnology in agriculture and forestry, vol. 10. Legumes and oilseed crops I, pp. 385–417. Springer-Verlag, Berlin.
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Inomata, N. Crossability and cytology of hybrid progenies in the cross between Brassica campestris and three wild relatives of B. oleracea, B. bourgeaui, B. cretica and B. montana . Euphytica 69, 7–17 (1993). https://doi.org/10.1007/BF00021721
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DOI: https://doi.org/10.1007/BF00021721