Summary
Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.
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Hille, J., Verheggen, F., Roelvink, P. et al. Bleomycin resistance: a new dominant selectable marker for plant cell transformation. Plant Mol Biol 7, 171–176 (1986). https://doi.org/10.1007/BF00021328
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DOI: https://doi.org/10.1007/BF00021328