Abstract
We have used RNA gel blot analysis to demonstrate the anther-specific expression of three genes in sunflower. Expression of these genes was first detected shortly before flower opening, which occurs sequentially on the sunflower inflorescence, and continues during pollination. In contrast, these genes are not expressed (or only weakly expressed) in a male-sterile line in which anther development aborts. In situ hybridization experiments showed that these genes are only expressed in the single cell layer of the sunflower anther epidermis. In the case of one of these genes, which codes for an abundant mRNA, we report the peptide sequences deduced from the sequence of two similar but non identical cDNAs. These proteins contain a potential signal peptide and are characterized by the presence of a proline-rich region which reads KPSTPAPPPPPP(PP)K. Our results also suggest that several proline-rich proteins of unknown functions are specifically synthesized during the maturation of anthers in sunflower.
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References
Arrand JE: Preparation of nucleic acid probes. In: Hames BD, Higgins SJ (eds) Nucleic Acid Hybridisation: A Practical Approach, pp. 17–45. IRL Press, Oxford, Washington (1985).
Bernier G: The control of floral evocation and morphogenesis. Ann Rev Plant Physiol Plant Mol Biol 39: 175–219 (1988).
Bohlmann H, Clausen S, Behnke S, Hiller C, Reimann-Philip U, Schrader G, Barkholt V, Apel K: Leaf-specific thionins of barley — a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants. EMBO J 7: 1559–1565 (1988).
Bowman JL, Smyth DR, Meyerowitz EM: Genes directing flower development in Arabidopsis. Plant Cell 1: 37–52 (1989).
Cassab GI, Varner JE: Cell wall proteins. Ann Rev Plant Physiol 39: 321–353 (1988).
Chen J, Varner JE: Isolation and characterization of cDNA clones for carrot extensin and a proline-rich 33-kDa protein. Proc Natl Acad Sci USA 82: 4399–4403 (1985).
Datta K, Schmidt A, Marcus A: Characterization of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes. Plant Cell 1: 945–952 (1989).
Domon C, Eyrard JL, Herdenberger F, Pillay DTN, Steinmetz A: Nucleotide sequence of two anther-specific cDNAs from sunflower (Helianthus annuus L.). Plant Mol Biol 15: 643–646 (1990).
Feldman KA, Marks MD, Christianson MI, Quatrano RS: A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science 243: 1351–1354 (1989).
Franssen HK, Nap JP, Gloudemans T, Stiekema W, VanDam H, Govers F, Louwerse J, VanKammen A, Bisseling T: Characterization of cDNA for nodulin-75 of soybean: a gene product involved in early stages of root nodule development. Proc Natl Acad Sci USA 84: 4495–4499 (1987).
Goldberg RB: Plants: novel developmental processes. Science 240: 1460–1467 (1988).
Hanson DD, Hamilton DA, Travis JL, Bashe DM, Mascarenhas JP: Characterization of a pollen-specific cDNA clone from Zea mays and its expression. Plant Cell 1: 173–179 (1989).
Haughn GW, Somerville CR: Genetic control of morphogenesis in Arabidopsis. Dev Genet 9: 73–89 (1988).
Herdenberger F, Evrard JL, Kuntz M, Tessier LH, Klein A, Steinmetz A, Pillay DTN: Isolation of flower-specific cDNA clones from sunflower. Plant Sci 69: 111–122 (1990).
Hong JC, Nagao RT, Key JL: Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1: 937–943 (1989).
Komaki M, Okada K, Nishino E, Shimura Y: Isolation and characterization of novel mutants of Arabidopsis thaliana defective in flower development. Development 104: 195–203 (1988).
Kuhlemeier C, Green PJ, Chua NH: Regulation of gene expression in higher plants. Ann Rev Plant Physiol 38: 221–257 (1987).
Kuntz M, Evrard JL, d'Harlingue A, Weil JH, Camara B: Expression of plastid and nuclear genes during chromoplast differentiation in bell pepper (Capsicum annuum) and sunflower (Helianthus annuus). Mol Gen Genet 216: 156–163 (1989).
Locker J: Analytical and preparative electrophoresis of RNA in agarose-urea. Anal Biochem 98: 358–367 (1979).
Meeks-Wagner DR, Dennis ES, ThanVan KT, Peacock WJ: Tobacco genes expressed during in vitro floral initiation and their expression during normal plant development. Plant Cell 1: 25–35 (1989).
Meyerowitz EM: In situ hybridization to RNA in plant tissue. Plant Mol Biol Rep 5: 242–250 (1987).
Momma T, Negoro T, Hirano M, Matsumoto A, Udaka K, Fukasawa C: Glycinin A5-A4-B3 mRNA: cDNA cloning and nucleotide sequencing of a splitting storage protein subunit of soybean. Eur J Biochem 149: 491–496 (1985).
Shotwell MA, Larkins BA: Biochemistry and molecular biology of seed storage proteins. In: Stumpf PK, Conn EE (eds) Biochemistry of Plants, Vol. 15, pp. 297–345. Academic Press, New York (1989).
Showalter AM, Varner JE: Plant hydroxyproline-rich glycoproteins. In: Stumpf PK, Conn EE (eds) Biochemistry of Plants, Vol. 15, pp. 485–520. Academic Press, new York (1989).
Smith AG, Hinchee MA, Horsch RB: Cell and tissue specific expression localized by in situ hybridization in floral tissues. Plant Mol Biol Rep 5: 237–241 (1987).
Sommer H, Beltran JP, Huijser P, Pape H, Lönnig WE, Saedler H, Schwartz-Sommer Z: Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors. EMBO J 9: 605–613 (1990).
Stinson JR, Eisenberg AJ, Willing RP, Pe ME, Hanson DD, Mascarenhas JP: Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol 83: 442–447 (1987).
Twell D, Wing R, Yamaguchi J, McCormick S: Isolation and expression of an anther-specific gene from tomato. Mol Gen Genet 217: 240–245 (1989).
Ursin VM, Yamaguchi J, McCormick S: Gametophytic and sporophytic expression of anther-specific genes in developing tomato anthers. Plant Cell 1: 727–736 (1989).
VanTunen AJ, Hartman SA, Mur LA, Mol JN: Regulation of chalcone flavonone isomerase (CHI) gene expression in Petunia hybrida: the use of alternative promoters in corolla, anthers and pollen. Plant Mol Biol 12: 539–551 (1989).
Zhang H, Scholl R, Browse R, Somerville C: Double stranded DNA sequencing as a choice for DNA sequencing. Nucl Acids Res 16: 1220 (1988).
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Evrard, JL., Jako, C., Saint-Guily, A. et al. Anther-specific, developmentally regulated expression of genes encoding a new class of proline-rich proteins in sunflower. Plant Mol Biol 16, 271–281 (1991). https://doi.org/10.1007/BF00020558
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DOI: https://doi.org/10.1007/BF00020558