Abstract
Numerous Arabidopsis genes have been cloned that correspond to putative pathogen defense-related genes identified in parsley (Petroselinum crispum). Treatment of Arabidopsis cells with fungal elicitor leads to rapid accumulation of the respective mRNAs with time courses comparable to those observed for their counterparts in parsley. Evolutionary sequence conservation of many of these genes in several plant species suggests they code for important plant functions.
Article PDF
Avoid common mistakes on your manuscript.
References
Dixon RA, Harrison MJ: Activation, structure, and organization of genes involved in microbial defense in plants. Adv Genet 28: 165–234 (1990).
Bowles DJ: Defense-related proteins in higher plants. Annu Rev Biochem 59: 873–907 (1990).
Hahlbrock K, Scheel D: Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Mol Biol 40: 347–369 (1989).
Somssich IE, Bollmann J, Hahlbrock K, Kombrink E, and Schulz W. Differential early activation of defenserelated genes in elicitor-treated parsley cells. Plant Mol Biol 12: 227–234 (1989).
Schmelzer E, Krüger-Lebus S, Hahlbrock K: Temporal and spatial patterns of gene expression around sites of attempted fungal infection in parsley leaves. Plant Cell 1: 993–1001 (1989).
Murray MG, Thompson WF: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 6323–6327 (1980).
Lois R, Dietrich A, Hahlbrock K, Schulz W: A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J 8: 1641–1648 (1989).
Somssich IE, Schmelzer E, Bollmann J, Hahlbrock K: Rapid activation by fungal elicitor of genes encoding ‘pathogenesis-related’ proteins in cultured parsley cells. Proc Natl Acad Sci USA 83: 2427–2430 (1986).
Kawalleck P: Pathogenresistenz in Planzen: Identifizierung von Genen, deren Produkte an induzierten Abwehrreaktionen in Petersilie beteiligt sind. Dissertation, University of Cologne, FRG (1991).
Kawalleck P, Plesch G, Hahlbrock K, Somssich IE: Induction by fungal elicitor of S-adenosyl-L-methionine synthetase and S-adenosyl-L-homocysteine hydrolase mRNAs in cultured cells and leaves of Petroselinum crispum. Proc Natl Acad Sci USA 89: 4713–4717 (1992).
Douglas C, Hoffmann H, Schulz W, Hahlbrock K: Structure and elicitor or u.v.-light-stimulated expression of two 4-coumarate: CoA ligase genes in parsley. EMBO J 6: 1189–1195 (1987).
Reimold U, Kroger M, Kreuzaler F, Hahlbrock K: Coding and 3′ non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme. EMBO J 2: 1801–1805 (1983).
Feinbaum RL, Ausubel FM: Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol Cell Biol 8: 1985–1992 (1988).
Davis KR, Schott H, Dong X, Ausubel FM: Arabidopsis thaliana as a model system for studying plant-pathogen interactions. In: Lugtenberg BJJ (ed) Signal Molecules in Plants and Plant-Microbe Interactions, pp. 99–106. Springer-Verlag, Berlin (1989).
Dangl JL, Lehnacker H, Kiedrowski S, Debener T, Rupprecht C, Arnold M, Somssich IE: Interactions between Arabidopsis thaliana and phytopathogenic Pseudomonas pathovars: a model for the genetics of disease resistance. In: Hauke H, Verma DPS (eds) Current Plant Science and Biotechnology in Agriculture, Vol. 1: Advances in Molecular Genetics of Plant-Microbe Interactions, pp. 84–89. Kluwer Academic Publishers, Dordrecht (1991).
Koch E, Slusarenko A: Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell 2: 437–445 (1990).
Roberts E, Kutchan T, Kolattukudy PE: Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits. Plant Mol Biol 11: 15–26 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Trezzini, G.F., Horrichs, A. & Somssich, I.E. Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells. Plant Mol Biol 21, 385–389 (1993). https://doi.org/10.1007/BF00019954
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00019954