Abstract
The cyanobacteria Synechocystis 6803 and 6714 contain three genes (psbA) coding for the D1 protein. This protein is an essential subunit of photosystem II (PSII) and is the target for herbicides. We have used herbicide-resistant mutants to study the role of the two homologous copies of the psbA genes in both strains (the third copy is not expressed). Several herbicide resistance mutations map within the psbAI gene in Synechocystis 6714 (G. Ajlani et al.), Plant Mol. Biol. 13 (1989): (469–479). We have looked for mutations in copy II. Results show that in Synechocystis 6714, only psbAI contains herbicide resistance mutations. Relative expression of psbAI and psbAII has been measured by analysing the proportions of resistant and sensitive D1 in the thylakoid membranes of the mutants. In normal growth conditions, 95% resistant D1 and 5% sensitive D1 were found. In high light conditions, expression of psbAII was enhanced, producing 15% sensitive D1. This enhancement is specifically due to high light and not to the decrease of D1 concentration caused by photoinhibition. Copy I of Synechocystis 6714 corresponds to copy 2 of Synechocystis 6803 since it was always psbA2 which was recombined in Synechocystis 6803 transformants. PSII of the transformant strains was found to be 95% resistant to herbicides as in resistant mutants of Synechocystis 6714.
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Ajlani G, Kirilovsky D, Picaud M, Astier C: Molecular analysis of psbA mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714. Plant Mol Biol 13: 469–479 (1989).
Ajlani G, Meyer I, Vernotte C, Astier C: Mutation in phenol-type herbicide resistance maps within the psbA gene in Synechocystis 6714. FEBS Lett 246: 207–210 (1989).
Ajlani G, Meyer I, Astier C, Vernotte C: Inhibition of photosystem II by ioxynil in wild type and resistant mutant of Synechocystis 6714. Z Naturforsch 44c: 979–984 (1989).
Brusslan J, Haselkorn R: Resistance to the photosystem II herbicide diuron is dominant to sensitivity in the cyanobacterium Synechococcus sp. PCC 7942. EMBO J 8: 1237–1245 (1989).
Bustos SA, Schaffer MR, Golden SS: Different and rapid responses of four cyanobacterial psbA transcripts to changes in light intensity. J Bact 172: 1998–2004 (1990).
Crejzet S, Ajlani G, Vernotte C, Astier C: A new ioxynilresistant mutant in Synechocystis PCC 6714: Hypothesis on the interaction of ioxynil with the D1 protein. Z Naturforsch 45c: 436–440 (1990).
Golden SS, Brusslan J, Haselkorn R: Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J 5: 2789–2798 (1986).
Gurevitz M, Osiewacz HD, Keren Y: Molecular evidence for interchromosomal recombination in the cyanobacterium Synechocystis sp. PCC 6803. Plant Sci 78: 217–224 (1991).
Herdman M, Delaney SF, Carr NG: A new medium for the isolation and growth of auxotrophic mutants of the blue-green alga Anacystis nidulans. J Gen Microbiol 79: 233–237 (1973).
Jansson C, Debus RJ, Osiewacz HD, Gurevitz M, McIntosh L: Construction of an obligate photoheterotrophic mutant of the cyanobacterium Synechocystis 6803. Plant Physiol 85: 1021–1025 (1987).
Kirilovsky D, Ajlani G, Picaud M, Etienne AL: Mutations responsible for high light sensitivity in an atrazineresistant mutant of Synechocystis 6714. Plant Mol Biol 13: 355–363 (1989).
Kirilovsky D, Ducruet JM, Etienne AL: Primary events occurring in photoinhibition in Synechocystis 6714 wild-type and atrazine-resistant mutant. Biochim Biophys Acta 1020: 87–93 (1990).
Kirilovsky D, Vernotte C, Etienne AL: Protection from photoinhibition by low temperature in Synechocystis 6714 and in Chlamydomonas reinhardtii: Detection of an intermediary state. Biochemistry 29: 8100–8106 (1990).
Krupa Z, Oquist G, Gustafsson P: Photoinhibition and recovery of photosynthesis in psbA gene-inactivated strains of cyanobacterium Anacystis nidulans. Plant Physiol 93: 1–6 (1990).
Krupa Z, Oquist G, Gustafsson P: Photoinhibition of photosynthesis and growth responses at different light levels in psbA gene mutants of the cyanobacterium Synechococcus. Physiol Plantarum 82: 1–8 (1991).
Metz J, Nixon P, Diner B Nucleotide sequence of the psbA3 gene from the cyanobacterium Synechocystis PCC 6803. Nucl Acids Res 18: 6715 (1991).
Mohamed A, Jansson C: Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol 13: 693–700 (1989).
Osiewacz HD, McIntosh L: Nucleotide sequence of a member of the psbA multigene family from the unicellular cyanobacterium Synechocystis 6803. Nucl Acids Res 15: 10585 (1987).
Perewoska I, Vernotte C, Picaud M, Astier C: Mutations in the D1 subunit of photosystem II and resistance to the phenol type herbicide ioxynil in Synechocystis PCC 6714 and 6803. Z. Naturforsch. 47c: 580–584 (1992).
Ravnikar PD, Debus R, Sevrinck J, Saetaert P, McIntosh L: Nucleotide sequence of a second psbA gene from the unicellular cyanobacterium Synechocystis 6803. Nucl Acids Res 17: 3991 (1989).
Robinson H, Golden SS, Brusslan J, Haselkorn R: Functioning of photosystem II in mutant strains of the cyanobacterium Anacystis nidulans R2. In: Biggins (ed.) Progress in Photosynthesis Research vol 4, pp 825–828. Martinus Nijhoff Publishers, The Hague (1987).
Schaefer MR, Golden MR: Differential expression of members of a cyanobacterial psbA gene family in response to light. J Bact 171: 3973–3981 (1989).
Schaefer MR, Golden SS: Light availability influences the ratio of two forms of D1 in cyanobacterial thylakoids. J Biol Chem 13: 7412–7417 (1989).
Trebst A: The molecular basis of resistance of photosystem II herbicides. In: Caseley JC, Cussans GW, Atkin RK (eds) Herbicide Resistance in Weeds and Crops, pp. 145–163. Butterworth-Heinemann, Oxford (1991).
Vernotte C, Astier C, Olive J: State 1-state 2 adaptation in the cyanobacteris Synechocystis PCC 6803 wild-type and phycocyanin-less mutant. Photosynthesis Res 26: 203–212 (1990).
Vrba J, Curtis SE: Characterization of a four-member psbA gene family from the cyanobacterium Anabaena 7120. Plant Mol Biol 14: 81–92 (1989).
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Bouyoub, A., Vernotte, C. & Astier, C. Functional analysis of the two homologous psbA gene copies in Synechocystis PCC 6714 and PCC 6803. Plant Mol Biol 21, 249–258 (1993). https://doi.org/10.1007/BF00019941
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DOI: https://doi.org/10.1007/BF00019941