Abstract
The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the α-insert) that is present in the cob gene of C. smithii. The α-insert, a group I intron (Cs cob·1) containing an open reading frame (ORF) which encodes a basic, hydrophilic protein of 237 amino acids, is unidirectionally transmitted to all diploid progeny during interspecific crosses. In this report, we show that the Cs cob·1-encoded protein is a site-specific endonuclease (I-Csm I) which could mediate the intron transfer via the gene conversion mechanism. The Cs cob·1 ORF was cloned into the vector pMALcr1 and over-expressed as a hybrid protein fused to maltose-binding protein (MBP). This fusion protein exhibited an in vivo endonuclease activity which specifically cleaved the intron homing site within the intronless cob gene.
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Ma, DP., King, YT., Young, K. et al. The group I intron of apocytochrome b gene from Chlamydomonas smithii encodes a site-specific endonuclease. Plant Mol Biol 18, 1001–1004 (1992). https://doi.org/10.1007/BF00019218
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DOI: https://doi.org/10.1007/BF00019218